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Adv Biomed Res 2021,  10:3

Molecular typing of community-acquired methicillin-resistant Staphylococcus aureus isolated from 2- to 6-year old children by staphylococcal protein A and agr typing in Isfahan, Iran

1 Nosocomial Infection Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
2 Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
3 Department of Laboratory Sciences, Khomein University of Medical Sciences, Khomein, Iran

Date of Submission09-Jun-2020
Date of Acceptance28-Sep-2020
Date of Web Publication27-Jan-2021

Correspondence Address:
Dr. Davood Azadi
Department of Laboratory Sciences, Khomein University of Medical Sciences, Khomein
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/abr.abr_139_20

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Background: Methicillin - resistant Staphylococcus aureus (MRSA) has become a considerable public health concern in the entire world due to the rapid spread of this bacterium in human community; also the epidemiology of MRSA has changed, as the isolation of MRSA strains from healthy and non-healthy patients. Therefore, the objective of this study is to determine the genetic diversity and antibiotic resistance profile of community-acquired (CA)-MRSA nasal carriage in the Iranian samples. Materials and Methods: A total of 25 CA-MRSA were isolated from the anterior nares of 410 healthy preschool children. All MRSA isolates were characterized by the detection of the toxic shock syndrome toxin-1 (TSST-1) and typed by γ-hemolysin genes, agr groups, and staphylococcal protein A (spa) typing. Kirby-Buyer antibiotic susceptibility testing was performed and interpreted as per the standard guidelines. Results: A total of 25 (6.1%) MRSA isolates were recovered from the anterior nares of 410 preschool children. Sixteen isolates (64%) were positive for the TSST-1 gene. Three agr specificity groups were determined, as follows: eight (32%) isolates belonged to agr Group I, five (20%) isolates belonged to agr Group II, and 12 (48%) isolates belonged to agr Group III. The repeated profiles of these spa types of 25 isolates were organized into eight different lineages groups. Five of lineages contained a single strain, three of lineages contained two strains, and three of lineages consisted of more than three strains. Conclusions: The results of our study show that the rate of MRSA in our region is significantly high. Additionally, spa type t037 was the predominant type among CA S. aureus.

Keywords: Agr protein, Methicillin-resistant Staphylococcus aureus, staphylococcal protein A, toxic shock syndrome toxin 1

How to cite this article:
Mobasherizadeh S, Shojaei H, Azadi D, Havaei SA, Ataei B, Khorvash F. Molecular typing of community-acquired methicillin-resistant Staphylococcus aureus isolated from 2- to 6-year old children by staphylococcal protein A and agr typing in Isfahan, Iran. Adv Biomed Res 2021;10:3

How to cite this URL:
Mobasherizadeh S, Shojaei H, Azadi D, Havaei SA, Ataei B, Khorvash F. Molecular typing of community-acquired methicillin-resistant Staphylococcus aureus isolated from 2- to 6-year old children by staphylococcal protein A and agr typing in Isfahan, Iran. Adv Biomed Res [serial online] 2021 [cited 2022 Aug 17];10:3. Available from:

  Introduction Top

Staphylococcus aureus, especially methicillin-resistant S. aureus (MRSA), is one of the most important bacterial pathogens in human and is responsible for the constantly increasing the number of community-acquired (CA) and nosocomial infections, including endocarditis, food poisoning, toxic shock syndrome, septicaemia, skin infections, soft-tissue infections, and bone infections.[1],[2] Furthermore, the appearance of CA-MRSA and the potential risks of its introduction into hospitals are the matters of great concern.[3] In the past few years, the isolation of CA-MRSA has been more frequent, especially in the geographical areas with a high prevalence, where these strains have also started to replace HA-MRSA in the hospital settings.[4] Epidemiological data on CA-MRSA carriage and infection and clonal diversity of CA-MRSA in our region are low.[4],[5]

S. aureus is a heterogeneous species that was recently found to have a clonal population structure and show a high degree of linkage disequilibrium (non-random associations between genetic loci).[6] The distribution of MRSA clonal types (CT) has been not well characterized across different geographic regions and care settings, especially in developing country.[7] Therefore, understanding the spread of specific CT of S. aureus, in both the hospitals and community settings, is of great importance for the prevention of this infection.

The use of adequate and accurate epidemiological typing methods is a prerequisite for screening and limiting the occurrence and spreading of dangerous clones within and between hospitals and community settings.[7],[8] Therefore, typing systems must enable the differentiation between unrelated isolates as well as the recognition of isolates belonging to the same clonal lineage in order to determine whether epidemiologically related isolates are also genetically related.[7]

Recently, similar to other bacteria, S. aureus typing has been dominated by the molecular techniques based on the various analysis of specific gene sequences in isolated strain.[7] Different molecular methods were used for typing of S. aureus such as staphylococcal cassette chromosome mec typing (SCCmec), multi-locus sequence typing (MLST), multilocus enzyme electrophoresis (MLEE), pulsed-field gel electrophoresis (PFGE), and staphylococcal protein A (spa).[9],[10] Among these methods, spa typing is a rapid, easy, and relatively inexpensive typing technique, it has shown effectively different genetic variation associated with CA-MRSA strains and they are associated with different geographical areas.[11]

Due to the literature in the current study, we aimed to determine the prevalence of the virulence genes, spa typing, and molecular characteristics of nasal carriage CA-MRSA isolates from healthy preschool children in Isfahan, Iran.

  Materials and Methods Top

Bacterial isolates

From December 2017 to September 2018 in a cross-sectional study conducted on 410 healthy 2-to 6-year-old preschool children in Isfahan, Iran, 25 CA-MRSA isolates were detected using the phenotypic biochemical test and the polymerase chain reaction (PCR) amplification mec A gene PCR.[4] In the present study, all of the 25 CA-MRSA isolates were included. This study was approved by the ethics committee of Isfahan University of Medical Sciences and Social Welfare Organization under which the private and public day-care nurseries (Grant No. 392063). A parent of any child participants provided informed consent on their behalf.

Ethics statement

This study was approved by the ethics committee of Isfahan University of Medical Sciences and Social Welfare Organization under which the private and public day-care nurseries or kindergartens are organized and operate (Grant No. 392062). A parent or guardian of any child participants provided informed consent on.

Antimicrobial-susceptibility testing

The recovered S. aureus strains were tested for antimicrobial susceptibility pattern to a panel of 16 antibiotic discs with the disk-diffusion technique on Mueller-Hinton agar (Mast, UK), and the results were recorded after incubation for 18 h at 37°C. The interpretive criteria for susceptibility were used by the clinical and laboratory standards institute.[12] The antimicrobial drugs tested included penicillin (PG 10 μg), clindamycin (CD 2 μg), ampicillin (AP 10 μg), vancomycin (VA 30 μg), levofloxacin (LEV 5 μg), teicoplanin (TEC 30 μg), ceftriaxone (CRO 30 μg), gentamicin (GM 10 μg), amikacin (AK 30 μg), tobramycin (TN10 μg), linezolid (LZD 30 μg), erythromycin (E 15 μg), kanamycin (K 30 μg), gatifloxacin (GAT 5 μg), ciprofloxacin (CIP 5 μg), and trimethoprim-sulfamethoxazole (TS 25 μg). Intermediate susceptibility was scored as resistance. Multidrug resistance was defined as resistance to three or more unique antibiotic classes in addition to beta-lactams. S. aureus ATCC25923 was used as a quality control strain in each test run.

Molecular assays

DNA extraction and purification

Chromosomal DNA of isolates was extracted using a simple boiling method. In summary, several colonies of bacteria were added to 200 ml TES buffer, (50 mM Tris hydrochloride (pH 8.0), 5 mM EDTA, 50 mM NaCl), and the suspension was heated at 95°C for 10 min and centrifuged at 11000 g for 10 min. The supernatant was transferred to another sterile microtube and centrifuged at 20 000 g for 10 min. The supernatant was resuspended in 50 μl Milli-Q water and stored at −20°C.

Toxic shock syndrome toxin and gene detection

The presence of Toxic Shock Syndrome Toxin-1 (TSST-1) genes in our isolates was determined using the specific PCR amplification strategy developed by Motoshima.[13]

Multiplex polymerase chain reaction for determination of agr group

The agr specific groups of CA-MRSA isolates were determined by 2 duplex PCR according to the methods of Saïd-Salim et al.[14]

Staphylococcal protein A typing

The polymorphic X region of the protein, a gene of all CA-MRSA isolates was determined by using the specific PCR protocol described by Strommenger et al.[15] Then, sequencing was performed by the Bioneer Company (South Korea), and the sequence data received were aligned manually with existing sequences of spa genes retrieved from the GenBank database. spa types were determined by using Ridom Staph-Type software (version 1.4; Ridom GmbH, Wurzburg, Germany), as described by Harmsen et al.[16] Finally, diversity and phylogenetic analyses of spa types of isolates were conducted with MEGA 8 software (version 8.1; Penn State University , Pennsylvania , USA

  Results Top

In this study, total of 25 (6.1%) MRSA isolates were recovered from the anterior nares of 410 preschool children. Among the 25 CA-MRSA isolates, 16 isolates (64%) were positive for the TSST-1 gene. The antimicrobial susceptibility pattern was observed among our isolates given in [Table 1].
Table 1: Antimicrobial susceptibility pattern and distribution of staphylococcal protein A types agr group and tst in 24 multidrug resistance isolates from healthy children in Isfahan

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  Agr groups Top

In the current study, three agr specificity groups were determined as follows: 8 (32%) isolates belonged to agr Group I, 5 (20%) isolates belonged to agr Group II, and 12 (48%) isolates belonged to agr group III [Table 1].


To control the intra-laboratory reproducibility of the sequence-based typing method, every 25 isolates in this study was typed repeatedly. All previous typing results could be confirmed, thus leading to an intra-laboratory reproducibility of 100%.

Diversity of staphylococcal protein A types

As described in the literature,[16] a spa type is composed of various repeats, each of which represents eight codons (24 nucleotides). In this study, a total of 25 distinct spa types were observed from 25 CA-MRSA strains. The repeat profiles of these spa types of 25 isolates were organized into eight different lineage groups [Figure 1]. Five of lineages contained a single strain, 3 of lineages contained two strains, and 3 of lineages consisted of more than three strains.
Figure 1: Staphylococcal protein A type sequence-based phylogenetic tree for the methicillin-resistantS. aureus isolates, produced using the neighbor-joining method by staph Type software. The number at each node represent the bootstrapping values

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  Discussion Top

Due to the advances in bacterial genomics, DNA sequencing, and bioinformatics have enriched the molecular tools of population geneticists, evolutionary biologists, and infection control facility to track and control the suspected outbreaks. MLEE, MLVA, PFGE, and MLST have been the most common methods for the application in population geneticists and those studying global epidemiology because these techniques accumulate genetic variation slowly.[17]

Different genotyping techniques are available for classifying S. aureus strains for epidemiological investigation, including “band-based” as well as “sequence-based” methods. Thereby, sequence-based typing methods, such as spa typing and MLST, have several obvious advantages, for instance they are easy to use, transportable, reproducible, and they can provide comparable results, compared to band-based methods, such as small macro restriction analysis.[11],[17] Due to the issue, the aim of the current study was the evaluation of the spa typing use as an appropriate tool for the routine typing of staphylococcal isolates in the clinical laboratory reference center for staphylococcus isolates, in accordance with previously proposed guidelines.

The repeated profiles of these spa types of 25 isolates were organized into eight different lineage groups. Lineages were formed by grouping strains together with similar spa repeated profiles, which presents the accumulation of identical point mutations, suggesting a genetic relatedness and descent from a common ancestor [Figure 1].[18] Same spa profile groupings were obtained by utilizing a multiple-sequence alignment program (MEGA 8). As an example, although spa type 46 (YMBQBLO) differed in the number of repeats from spa type 7 (YHGCMBQBLO), these two were grouped together in spa lineage 1 because they contained many identical repeats representing exact nucleotide polymorphisms shared by both.

In the current study, we found that 25 different spa types such as t037, t030, t7685, t7689, t030, t3096, that correspond with the results of other studies in Iran such as studies conducted by Mirzaii et al. and Japoni-Nejad et al. that were isolated and spa typed MRSA from personnel, patients, air, and intensive care unit in Iran in 2014.[19],[20] These result showed that the spa types t037, t030, t7685, and t7689 were the most common spa type our region.

In correlation with the results of previous studies, we found that spa typing has a high degree of typeability and reproducibility in our laboratory.[21] The unambiguous nomenclature also facilitates the submission of comparable typing information to international networks, which is in clear contrast to other methods based on fragment patterns, such as PFGE, that have the lack of interlaboratory reproducibility. Thus, spa typing can be an excellent tool for the international multicenter surveillance of MRSA strains.[21],[22]

The typing data resulting from those studies can also be used to enlarge our knowledge of spa-MLST mapping, which is extremely useful for the daily routine typing of MRSA, in which the Based Upon Repeat Pattern (BURP) algorithm together with “reference spa types” enable the classification of isolates into particular clonal lineages. This work, as well as previous studies, have shown that, in general, the typing concordance between spa typing-BURP analysis and alternative methods is high; however, the occurrence of “group violations”[16] associated with particular BURP groups and clonal lineages were also demonstrated. Some of these misclassifications (in BURP groups A, E, and G) are due to the related spa repeat successions in isolates of different clonal lineages, possibly caused by the recombination events in the spa.

The successful application of spa typing for the detection of transmission events or clusters of infections was reported in different studies.[11],[23] The results of our study are able to monitor clusters of infections over an even longer period of time and also have a correlation with the in vivo stability of the spa locus as a molecular marker in the epidemiological research.[24] The endemic spread of these highly successful types finally leads to a lack of discrimination in local site epidemiology. To overcome this limitation, recent studies suggest the use of a combination of different typing techniques to increase the ability to discriminate isolates.[25] We have previously described the use of a combination of two techniques (SCC mec and MLST analysis) for the successful subtyping of isolates.[4]

In our study, the majority of MRSA strains recovered from specimens were agr group I, which is consistent with previous reports. For example, Shopsin et al. 12 showed that agr specific group I (42%) was prevalent between children and their guardians, and in the van Leeuwen et al. 13 collection of 192 MRSA isolates, 71% of strains belonged to agr group I. Other study indicated a high prevalence of agr Group II in the nosocomial infection group.[26],[27] This is likely due to ecological and geographical structuring.

Considering the possible relation between the agr group and the antibiotic resistance in S. aureus isolates, we made disk-diffusion agar tests. Resistance pattern to oxacilin between the agr groups was relatively even. Agr-specific Group II isolates except oxacilin were susceptible to all antibiotic disks tested as opposed to the others, and agr-specific group IV isolates were more resistant strains.

MRSA can produce many exotoxins such as TSST-1 and α-hemolysin which were associated with a severe illness that includes shock and multiple organ failure and is called toxic shock syndrome.[28],[29] Approximately 20% and 5% of MRSA isolates possess the genes encoding TSST-1, and α-hemolysin, respectively. However, the result of the current study showed that 16 isolates (64%) and 4 isolates (16.6%) were positive for the TSST-1 and α-hemolysin gene, respectively. This result showed that our CA-MRSA have high pathogenicity in compared to CA-MRSA of other regions.

  Conclusion Top

In conclusion, the authors demonstrated the value of spa typing in combination with agr groups of the isolates as a suitable and reproducible tool for routine epidemiological typing based on a random sampling of isolates. The results of our study show that this approach yields highly interchangeable and reproducible information that may be used both for local epidemiology and for national as well as international surveillance of MRSA isolates. However, to overcome the limitations of a single locus-based molecular typing method, the use of additional markers is indispensable. To reduce time and cost, those markers should be selected on the basis of the clonal lineage inferred by spa typing and also on the basis of the question to be addressed. Additional targets can be SCCmec, agr groups, resistance genes or lineage-specific virulence gene, or alternative polymorphic regions of the S. aureus chromosome.


We would like to thank the Infectious Diseases and Tropical Medicine Research Center laboratory staff for supporting the practical work.

Financial support and sponsorship

This work was financially supported by the deputy vice-chancellor for research affairs of Isfahan University of Medical Sciences (grant numbers: 392062).

Conflicts of interest

There are no conflicts of interest.

  References Top

Peters J, Price J, Llewelyn M. Staphylococcal and streptococcal infections. Medicine 2017;45:727-34.  Back to cited text no. 1
Reddy PN, Srirama K, Dirisala VR. An update on clinical burden, diagnostic tools, and therapeutic options of Staphylococcus aureus. Infect Dis (Auckl) 2017;10:1179916117703999.  Back to cited text no. 2
Heudorf U, Albert-Braun S, Hunfeld KP, Birne FU, Schulze J, Strobel K, et al. Multidrug-resistant organisms in refugees: Prevalences and impact on infection control in hospitals. GMS Hyg Infect Control 2016;11:Doc16.  Back to cited text no. 3
Mobasherizadeh S, Shojaei H, Azadi D, Havaei SA, Rostami S. Molecular characterization and genotyping of methicillin-resistant Staphylococcus aureus in nasal carriage of healthy Iranian children. J Med Microbiol 2019;68:374-8.  Back to cited text no. 4
Poormohammadi S, Farahani A, Mohajeri P. Genomic diversity and antimicrobial susceptibility profiling of nasal carriage Staphylococcus aureus isolated from pediatric ward in Western Iran. Saudi J Biol Sci 2019;26:1-7.  Back to cited text no. 5
Pires Dos Santos T, Damborg P, Moodley A, Guardabassi L. Systematic review on global epidemiology of methicillin-resistant Staphylococcus pseudintermedius: Inference of population structure from multilocus sequence typing data. Front Microbiol 2016;7:1599.  Back to cited text no. 6
Coleman DC, Shore AC, Goering RV, Monecke S. Editorial: New Insights and updates on the molecular epidemiology and antimicrobial resistance of MRSA in humans in the whole-genome sequencing era. Front Microbiol 2019;10:637.  Back to cited text no. 7
Mohamed N, Timofeyeva Y, Jamrozy D, Rojas E, Hao L, Silmon de Monerri NC, et al. Molecular epidemiology and expression of capsular polysaccharides in Staphylococcus aureus clinical isolates in the United States. PLoS One 2019;14:e0208356.  Back to cited text no. 8
Abdulgader SM, Shittu AO, Nicol MP, Kaba M. Molecular epidemiology of Methicillin-resistant Staphylococcus aureus in Africa: A systematic review. Front Microbiol 2015;6:348.  Back to cited text no. 9
Lakhundi S, Zhang K. Methicillin-resistant Staphylococcus aureus: Molecular characterization, evolution, and epidemiology. Clin Microbiol Rev 2018;31.4:1-103.  Back to cited text no. 10
O'Hara FP, Suaya JA, Ray GT, Baxter R, Brown ML, Mera RM, et al. spa Typing and Multilocus Sequence Typing Show Comparable Performance in a Macroepidemiologic Study of Staphylococcus aureus in the United States. Microb Drug Resist 2016;22:88-96.  Back to cited text no. 11
Wayne P. Performance standards for antimicrobial susceptibility testing. USA Clinical and Laboratory Standards Institute (CLSI); 2017. p. 20.  Back to cited text no. 12
Motoshima M, Yanagihara K, Morinaga Y, Matsuda J, Sugahara K, Yamada Y, et al. Genetic diagnosis of community-acquired MRSA: A multiplex real-time PCR method for Staphylococcal cassette chromosome mec typing and detecting toxin genes. Tohoku J Exp Med 2010;220:165-70.  Back to cited text no. 13
Saïd-Salim B, Mathema B, Braughton K, Davis S, Sinsimer D, Eisner W, et al. Differential distribution and expression of Panton-Valentine leucocidin among community-acquired methicillin-resistant Staphylococcus aureus strains. J Clin Microbiol 2005;43:3373-9.  Back to cited text no. 14
Strommenger B, Kehrenberg C, Kettlitz C, Cuny C, Verspohl J, Witte W, et al. Molecular characterization of methicillin-resistant Staphylococcus aureus strains from pet animals and their relationship to human isolates. J Antimicrob Chemother 2006;57:461-5.  Back to cited text no. 15
Harmsen D, Claus H, Witte W, Rothgänger J, Claus H, Turnwald D, et al. Typing of methicillin-resistant Staphylococcus aureus in a university hospital setting by using novel software for spa repeat determination and database management. J Clin Microbiol 2003;41:5442-8.  Back to cited text no. 16
Pérez-Losada M, Cabezas P, Castro-Nallar E, Crandall KA. Pathogen typing in the genomics era: MLST and the future of molecular epidemiology. Infect Genet Evol 2013;16:38-53.  Back to cited text no. 17
Koreen L, Ramaswamy SV, Graviss EA, Naidich S, Musser JM, Kreiswirth BN. spa typing method for discriminating among Staphylococcus aureus isolates: Implications for use of a single marker to detect genetic micro- and macrovariation. J Clin Microbiol 2004;42:792-9.  Back to cited text no. 18
Mirzaii M, Emaneini M, Jabalameli F, Halimi S, Taherikalani M. Molecular investigation of Staphylococcus aureus isolated from the patients, personnel, air and environment of an ICU in a hospital in Tehran. J Infect Public Health 2015;8:202-6.  Back to cited text no. 19
Japoni-Nejad A, Rezazadeh M, Kazemian H, Fardmousavi N, van Belkum A, Ghaznavi-Rad E. Molecular characterization of the first community-acquired methicillin-resistant Staphylococcus aureus strains from Central Iran. Int J Infect Dis 2013;17:e949-54.  Back to cited text no. 20
Strommenger B, Braulke C, Heuck D, Schmidt C, Pasemann B, Nübel U, et al. spa Typing of Staphylococcus aureus as a frontline tool in epidemiological typing. J Clin Microbiol 2008;46:574-81.  Back to cited text no. 21
van Belkum A, Tassios PT, Dijkshoorn L, Haeggman S, Cookson B, Fry NK, et al. Guidelines for the validation and application of typing methods for use in bacterial epidemiology. Clin Microbiol Infect 2007;13 Suppl 3:1-46.  Back to cited text no. 22
Bonnet I, Millon B, Meugnier H, Vandenesch F, Maurin M, Pavese P, et al. High prevalence of spa type t571 among methicillin-susceptible Staphylococcus aureus from bacteremic patients in a French University Hospital. PLoS One 2018;13:e0204977.  Back to cited text no. 23
Goudarzi M, Seyedjavadi SS, Nasiri MJ, Goudarzi H, Sajadi Nia R, Dabiri H. Molecular characteristics of methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from patients with bacteremia based on MLST, SCCmec, spa, and agr locus types analysis. Microb Pathog 2017;104:328-35.  Back to cited text no. 24
Mehndiratta PL, Bhalla P. Typing of methicillin resistant Staphylococcus aureus: A technical review. Indian J Med Microbiol 2012;30:16-23.  Back to cited text no. 25
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Shopsin B, Mathema B, Alcabes P, Said-Salim B, Lina G, Matsuka A, et al. Prevalence of agr specificity groups among Staphylococcus aureus strains colonizing children and their guardians. J Clin Microbiol 2003;41:456-9.  Back to cited text no. 26
van Leeuwen W, van Nieuwenhuizen W, Gijzen C, Verbrugh H, van Belkum A. Population studies of methicillin-resistant and -sensitive Staphylococcus aureus strains reveal a lack of variability in the agr D gene, encoding a staphylococcal autoinducer peptide. J Bacteriol 2000;182:5721-9.  Back to cited text no. 27
Sada R, Fukuda S, Ishimaru H. Toxic shock syndrome due to community-acquired methicillin-resistant Staphylococcus aureus infection: Two case reports and a literature review in Japan. IDCases 2017;8:77-80.  Back to cited text no. 28
Diep BA, Le VT, Visram ZC, Rouha H, Stulik L, Dip EC, et al. improved protection in a rabbit model of community-associated methicillin-resistant Staphylococcus aureus necrotizing pneumonia upon neutralization of leukocidins in addition to alpha-hemolysin. Antimicrob Agents Chemother 2016;60:6333-40.  Back to cited text no. 29


  [Figure 1]

  [Table 1]


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