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ORIGINAL ARTICLE
Adv Biomed Res 2022,  11:45

Practice and associated factors regarding foot care among diabetes mellitus patients attending a rural primary health center in South India


1 Department of Community Medicine, KAHERs J.N. Medical College, Belagavi, Karnataka, India
2 KLES Diabetes Centre, KLES Dr. Prabhakar Kore Hospital and Medical Research Centre, Belagavi, Karnataka, India
3 Department of Biotechnology and Microbiology, Karnatak University, Dharwad, Karnataka, India

Date of Submission20-Nov-2020
Date of Acceptance13-Oct-2021
Date of Web Publication30-May-2022

Correspondence Address:
Dr. Srihari Dhandapani
Department of Community Medicine, KAHERs J.N. Medical College, Belagavi, Karnataka
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/abr.abr_279_20

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  Abstract 


Background: Diabetes mellitus patients are more likely to undergo nontraumatic foot or leg amputations because they have decreased circulation to the feet or reduced sensation. This condition may cause ulcerations, infections, and delayed wound healing, leading to amputations. Adequate knowledge and practices regarding foot care will be vital in the prevention of diabetic foot complications. Hence, this study aimed to determine the level of knowledge and practices of foot care among diabetes mellitus patients attending a rural primary health center in Belagavi-South India. Materials and Methods: A cross-sectional study was conducted in the rural field practice area of J.N. Medical College, Belagavi, for 7 months (November 2019 to May 2020). A pretested questionnaire was administered to 461 diabetes patients attending the health center. A written informed consent was obtained from each participant. They were interviewed with a questionnaire consisting of 15 “yes” or “no” questions, each on foot care knowledge and practice. Results: The mean age of the study participants was 50.28 ± 9.48 years. The mean duration of diabetes was 10.69 ± 7.09 years. Majority, i.e., 296 (64.2%) participants showed satisfactory knowledge and 293 (63.6%) showed satisfactory practice. Only 97 (21%) had poor knowledge, 90 (19.5%) had poor practice, 68 (14.8%) patients had good knowledge, and 78 (16.9%) had good practice about diabetic foot care. Conclusions: The low level of knowledge and practice in patients with diabetes regarding foot care necessitates an educational program to increase awareness related to foot complications of diabetes, thereby motivating diabetic patients to incorporate foot care practices in their day-to-day routine.

Keywords: Complications, diabetes mellitus, diabetic foot, epidemiology, foot care, India, knowledge, practice, prevention and control, primary health center


How to cite this article:
Dhandapani S, Kambar S, Hiremath MB. Practice and associated factors regarding foot care among diabetes mellitus patients attending a rural primary health center in South India. Adv Biomed Res 2022;11:45

How to cite this URL:
Dhandapani S, Kambar S, Hiremath MB. Practice and associated factors regarding foot care among diabetes mellitus patients attending a rural primary health center in South India. Adv Biomed Res [serial online] 2022 [cited 2022 Aug 14];11:45. Available from: https://www.advbiores.net/text.asp?2022/11/1/45/346317




  Introduction Top


Diabetes mellitus is a group of metabolic diseases characterized by hyperglycemia resulting from defects in insulin secretion, insulin action, or both. The number of people with diabetes in India was 65 million in 2016.[1] By 2035, it is estimated to rise to 109 million diabetic patients in the country.[2] The incidence of diabetic foot will surge due to a rise in global prevalence of diabetes mellitus along with an increased life expectancy among patients with diabetes, which is comparable to the life expectancy in the general population.[3],[4] Diabetic foot affects approximately 15% and 25% of all diabetic patients globally and in India, respectively.[5],[6]

A systematic review from India showed that 50% of diabetic foot ulcers get infected necessitating hospitalization and 20% require lower-extremity amputation. Diabetic foot ulcers contribute to approximately 80% of all nontraumatic amputations.[5] Patients with diabetic foot complications spend significantly more than diabetic patients with no complications.[7] In addition, diabetic foot ulcers have a negative impact on patients' health-related as well as overall quality of life.[8],[9],[10] The practice of foot care measures such as daily foot washing and drying, daily foot examination, proper nail care, and footwear are important with regard to prevention and early detection of the expected complications. Knowledge about the above-mentioned foot care practices varies among studies. A previous study by Ralineba et al.,[11] in South Africa, has shown that majority of people have insufficient knowledge, while a study by Pavithra et al.[12] in Karnataka, India, showed that seven and four out of ten study participants had good awareness and practice scores about foot care, respectively.

The number of studies on knowledge and practices about foot care among patients with diabetes in India especially in rural areas is very few. Besides, majority of patients in the rural areas are unaware about the importance of foot care and its consequences because of an education gap and also, information regarding foot care is not provided by the concerned authorities in the rural settings. With this background, the present study was carried out to assess the level of knowledge and practices of foot care among diabetes mellitus patients attending a rural primary health center in Belagavi-South India.


  Materials and Methods Top


This was a cross-sectional study conducted among 461 diabetic patients who attended the outpatient department of primary health center at Kinaye which is a rural field practice area affiliated with Jawaharlal Nehru Medical College, KAHER University, Belagavi, Karnataka, between November 2019 and May 2020.

Universal sampling method was used to select patients attending the clinic for routine diabetic health checkup. Patients who were willing to participate and provided informed consent were recruited for the study.

Selection criteria

Patients were included if they (a) were diabetic patients more than 18 years of age and (b) had duration of diabetes mellitus for more than 6 months.

A pretested structured questionnaire developed by Hasnain et al. was used to interview the participants which consisted of 15 “yes” or “no” questions each on foot care knowledge and current self-care practices.[13] Each correct response was assigned 1 mark. Scores for knowledge and practice were determined to be good, satisfactory, or poor based on the median and interquartile range (IQR) values.

Compliance was measured using the Medication Adherence Questionnaire (MAQ) by Lavasa et al.[14] The MAQ contains the same four questions as the 4-item Morisky Medication Adherence Scale. The closed question format with “yes-saying” bias allows disclosure of nonadherence. A patient scores 1 point for each “Yes” answer. Scores of 0, 1, or 2 have been termed as good compliance and scores of 3 or 4 have been termed as poor compliance. Morisky Scale questionnaire is the quickest to administer and is also able to identify barriers to adherence due to its length. Because it has been validated in the broadest range of diseases and in patients with low literacy, it has been used in this study and is also the most widely used scale for research.

The median values obtained were 8 (IQR: 7–10) and 8 (IQR: 6–9) for level of knowledge and practices regarding foot care, respectively. Those who scored more than the third quartile were considered to have good knowledge (>10) and practice (>9), and below the first quartile were poor for knowledge (<7) and practice (<6). Scores between first and third quartiles signified satisfactory knowledge (7–10) and practice (6–9).

Primary outcome variables were level of knowledge and practices of foot care. Sociodemographic and clinical parameters were the primary explanatory variables.

Statistical analysis

Descriptive analysis was carried out using mean and standard deviation for quantitative variables and frequency and proportion for categorical variables. All quantitative variables were checked for normal distribution within each category of the explanatory variable by using visual inspection of histograms and normality Q-Q plots. Shapiro–Wilk test was also conducted to assess normal distribution. P > 0.05 was considered as normal distribution.

For normally distributed quantitative parameters, mean values were compared between the study groups using ANOVA. If statistically significant differences were found using ANOVA, appropriate post hoc tests (least significant difference/Bonferroni) were used to assess statistical significance of pair-wise comparisons.

Categorical outcomes were compared between the study groups using Chi-square test/Fisher's exact test, wherever appropriate. Association between quantitative explanatory and outcome variables was assessed by calculating Spearman's rank correlation coefficient, and scatter plot was used to represent the data. Regression analysis was performed to identify the risk factors related to the main outcomes. P < 0.05 was considered statistically significant. Data obtained were analyzed using IBM SPSS Statistics for Windows, Version 22.0 (IBM Corp., Armonk, NY, USA).[15]

Ethical approval

The JNMC Institutional Ethics Committee on Human Subjects Research (Ref: MDC/DOME/435) approved the study.


  Results Top


Sociodemographic characteristics of the study participants

We recruited a total of 461 participants for the study. The mean age was 50.28 ± 9.48 years, and 230 (49.89%) were female [Table 1]. A total of 421 (91.32%) participants had a minimum primary school education, 195 (42.30%) were of agriculture background and 70 (15.18%) were doing business. A total of 301 (65.29%) participants belonged to either socioeconomic class IV or V,[16] and 203 (44.03%) participants had a family history of diabetes mellitus and 390 (84.60%) had good compliance of taking medication for diabetes.
Table 1: Distribution of study participants according to sociodemographic characteristics (n=461)

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Practices and knowledge regarding foot care

A total of 392 (85%) participants were taking antidiabetic treatment regularly [Table 2]. The responses were good for daily washing of feet and drying after washing. A total of 110 (23.86%) patients walked barefoot, 223 (48.4%) did not know the proper technique to trim toenails, and 288 (62.5%) knew that keeping the skin of the feet soft was important to prevent dryness, but majority (84.2%) had no knowledge of the importance of using talcum powder to keep interdigital spaces dry. There was a striking difference for responses on daily change of socks, lotion application in the interdigital spaces, using of warm water, or checking the temperature of water before use.
Table 2: Distribution of level of knowledge and practice about foot care in the study population (n=461)

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The median total scores of knowledge were 8 with an IQR of 7–10. The results showed that 68 (14.8%) participants had good knowledge, 295 (64.2%) had satisfactory knowledge, and 97 (21%) had poor knowledge regarding foot care. The median total scores of practice were 8 with an IQR of 6–9. For practices regarding foot care, 294 (63.6%) had satisfactory and 90 (19.5%) had poor foot care. Seventy-nine (16.9%) participants had good practice.

Predictors of knowledge regarding foot care

The mean difference for age across level of knowledge was statistically significant (P < 0.001) [Table 3] and [Figure 1]. Out of the 461 participants, 10.6% of the female participants had good knowledge compared to 4.1% of male participants. Among 231 male participants, 112 (48.5%) had poor knowledge regarding foot care (P < 0.001) [Figure 2]. Among 172 (37.3%) participants who had scored poor in knowledge, 19 (47.5%) were illiterate, 91 (68.4%) had completed primary schooling, 85 (43.6%) were farmers, and 42 (60%) were in business. In addition, the duration of diabetes was longer for 33 out of 68 participants with good knowledge compared to that of the other groups (P = 0.004).
Table 3: Comparison of demographic variables across the level of knowledge regarding diabetic foot care (n=461)

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Figure 1: Bar diagram showing comparison of mean age across level of knowledge and practice regarding foot care (n = 461)

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Figure 2: Bar diagram showing comparison of gender across level of knowledge and practice regarding foot care (n = 461)

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Predictors of practice regarding foot care

Out of the 78 participants with good foot care practice, 71 (91%) had either high school education or were graduates [Table 4]. We found that patients with good foot care practices were female (P = 0.012), worked in private sector, or were retired from work (P = 0.001). On the other hand, no significant differences were found between the groups in terms of age, family history of diabetes, and duration of diabetes.
Table 4: Comparison of demographic variables across the level of practice regarding diabetic foot care (n=461)

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Univariate logistic regression analysis showed statistically significant associations for knowledge as well as practice regarding diabetic foot care for explanatory variables such as male gender, illiteracy, primary school level of education, and agriculture or business as occupation [Table 5]. The strongest association was found with socioeconomic class 5 (P < 0.001) as compared to baseline (socioeconomic class 1). There were no statistically significant associations observed for duration or family history of diabetes.

There was a strong positive correlation between knowledge and practice regarding foot care (R = 0.91, P < 0.001) [Figure 3].
Table 5: Univariate logistic regression analysis of factors associated with poor knowledge and poor practice of diabetic foot care in study population (n=461)

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Figure 3: Correlation between knowledge and practice about foot care (n = 461)

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  Discussion Top


We performed a study aimed to assess the level of knowledge and current practices of our diabetic patients regarding foot care. The average standard scores for foot care were lower for practice at 7.5 compared to knowledge which was 8.3. These findings are similar to a study done in Malaysia.[17]

The categories that scored poorly were checking the temperature and using warm water for washing/bathing, talcum powder usage, not applying lotion to keep interdigital spaces dry, and daily change of socks. Patients are generally advised to keep the skin of the feet soft by using moisturizing lotion or coconut oil. The low score obtained in keeping the interdigital spaces dry may be attributed to patients not knowing that lotion or oil should not be applied between the toes, and that talcum powder should be used instead. Not checking temperature or using hot or cold water for bathing in the place of warm water may be explained due to plausible factors such as habits, variations in weather, and personal preference. The low score in daily change of socks may be due to lack of proper information on hygiene. Patients must be informed about the ill effects of not checking the water so that scald burns can be averted especially in the lower extremities along with daily change of socks and keeping the interdigital spaces, often a neglected part of the feet, dry so that fungal infections can be prevented.[18]

Good practice for foot care was observed only in 79 (16.9%) participants, which is consistent with the results reported by Sutariya and Kharadi and Desalu et al.[19],[20] These data also showed that foot care practice was higher among patients who had good foot care knowledge, which is similar to a study done in Riyadh, Saudi Arabia.[21] The majority was of satisfactory practice score, which is similar to findings shown by Al-Asmary et al. in Saudi Arabia and Saber and Daoud in Iraq.[22],[23]

Almost one-fifth of the participants had poor foot care and their score of practice was almost always lower to the score of knowledge. This indicates reduced compliance and ignorance to the already-known benefits of foot care, which is consistent with findings from other studies.[13],[17],[20],[24],[25] Very few individuals had better foot care practice than knowledge, which may be attributed to support from family members' or patients' inclination to take control of their health.

The foot care scores increased from poor to good knowledge and practice with rise in the mean age, and our results were consistent with a study conducted by Saber and Daoud.[22] This may be because patients were receiving information regarding diabetes and foot care with frequent visits to the health center. In our study, the foot care practices of women were high compared to those of men, which is similar to findings from studies done in Colombia, Thailand, and Brazil.[25],[26] It may be because women may have better knowledge about the disease and, therefore, are more concerned about their health and well-being. Foot care score for practices were associated with occupation of the individuals, which coincides with the findings of other studies done in Iran.[27]

In our study, we noticed that as the duration of diabetes increased, the foot care knowledge increased, which was statistically significant. These findings are similar to a study documented by Li et al.[28] Although patients' knowledge increased with duration of disease, this knowledge was not converted into practice. These findings coincide with a study done by Gökdeniz et al.[29]

Our study revealed that patients with an educational level higher than primary schooling obtained noticeably higher foot care scores, the findings of which coincide with various studies.[13],[25],[30],[31],[32],[33] People with low literacy will have lesser knowledge and poorer practices. Most patients may not be receiving foot care education as part of the management of diabetes.

Limitations of the study

This was a cross-sectional study design wherein a causal relationship cannot be established. The results cannot be generalized for the entire country with multiple ethnicities because this was done at a single rural health center. Further research through a qualitative assessment may be required to assess the barriers to foot care in our developing country which has a high incidence of diabetes.

Recommendations

General public

Patient education and motivation to practice is an effective way to reduce the incidence of complications of diabetes and its management.

Family members should also be involved in the care to improve overall assistance, support, and motivation to the diabetic patients. Spouse or, more importantly, their children can be advised to escort them during regular checkups so that they can be taught, which would enable more compliance.

Health workers

Foot care education in a primary care setting is vital to reduce the incidence of diabetic foot complications.

Health-care workers can distribute educational pamphlets with pictorial illustrations of foot care and conduct camps to promote patients' knowledge and awareness.

Community health workers such as accredited social health activists (ASHA) who know and understand people can be given training in teaching and in diabetes management and they can be empowered to become diabetes educators also.


  Conclusions Top


We conclude that health-care professionals should teach about various complications which may come about without proper foot care. Patients with lower education need more information and knowledge about the diagnosis of diabetes and awareness regarding the complications. Patients will be motivated and empowered to include foot care into day-to-day routine when they understand the reasons behind its emphasis.

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.



 
  References Top

1.
India State-Level Disease Burden Initiative Diabetes Collaborators. The increasing burden of diabetes and variations among the states of India: The Global Burden of Disease Study 1990-2016. Lancet Glob Health 2018;6:e1352-62.  Back to cited text no. 1
    
2.
Guariguata L, Whiting DR, Hambleton I, Beagley J, Linnenkamp U, Shaw JE. Global estimates of diabetes prevalence for 2013 and projections for 2035. Diabetes Res Clin Pract 2014;103:137-49.  Back to cited text no. 2
    
3.
Tachkov K, Mitov K, Koleva Y, Mitkova Z, Kamusheva M, Dimitrova M, et al. Life expectancy and survival analysis of patients with diabetes compared to the non diabetic population in Bulgaria. PLoS One 2020;15:e0232815.  Back to cited text no. 3
    
4.
Zhang P, Lu J, Jing Y, Tang S, Zhu D, Bi Y. Global epidemiology of diabetic foot ulceration: A systematic review and meta-analysis. Ann Med 2017;49:106-16.  Back to cited text no. 4
    
5.
Ghosh P, Valia R. Burden of diabetic foot ulcers in India: Evidence landscape from published literature. Value Heal 2017;20:A485.  Back to cited text no. 5
    
6.
Schaper NC, Apelqvist J, Bakker K. The international consensus and practical guidelines on the management and prevention of the diabetic foot. Curr Diab Rep 2003;3:475-9.  Back to cited text no. 6
    
7.
Kumpatla S, Kothandan H, Tharkar S, Viswanathan V. The costs of treating long-term diabetic complications in a developing country: A study from India. J Assoc Physicians India 2013;61:102-9.  Back to cited text no. 7
    
8.
García-Morales E, Lázaro-Martínez JL, Martínez-Hernández D, Aragón-Sánchez J, Beneit-Montesinos JV, González-Jurado MA. Impact of diabetic foot related complications on the health related quality of life (HRQoL) of patients-A regional study in Spain. Int J Low Extrem Wounds 2011;10:6-11.  Back to cited text no. 8
    
9.
Alrub AA, Hyassat D, Khader YS, Bani-Mustafa R, Younes N, Ajlouni K. Factors associated with health-related quality of life among Jordanian patients with diabetic foot ulcer. J Diabetes Res 2019;2019:4706720.  Back to cited text no. 9
    
10.
Al Ayed M, Ababneh M, Alwin Robert A, Al Misfer N, Cruz M, Austria HC, et al. Factors associated with health-related quality of life in patients with diabetic foot ulcer: A cross-sectional study from Saudi Arabia. Cureus 2020;12:e8658.  Back to cited text no. 10
    
11.
Ralineba T, Netshikweta ML. Knowledge and practices associated with diabetes among patients with chronic diabetes mellitus in rural areas of Vhembe District, Limpopo Province, South Africa. J Hum Ecol 2015;51:193-201.  Back to cited text no. 11
    
12.
Pavithra H, Akshaya KM, Nirgude AS, Balakrishna AG. Factors associated with awareness and practice about foot care among patients admitted with diabetes mellitus: A cross sectional research from a medical college hospital of southern India. Nepal J Epidemiol 2020;10:897-904.  Back to cited text no. 12
    
13.
Hasnain S, Sheikh NH. Knowledge and practices regarding foot care in diabetic patients visiting diabetic clinic in Jinnah Hospital, Lahore. J Pak Med Assoc 2009;59:687-90.  Back to cited text no. 13
    
14.
Lavsa SM, Holzworth A, Ansani NT. Selection of a validated scale for measuring medication adherence. J Am Pharm Assoc (2003) 2011;51:90-4.  Back to cited text no. 14
    
15.
IBM Corp. IBM SPSS Statistics for Windows, Version 22.0. Armonk, NY: IBM Corp; 2013.  Back to cited text no. 15
    
16.
Pandey VK, Aggarwal P, Kakkar R. Modified BG Prasad socio-economic classification, update-2019. Indian J Community Heal 2019;31:123-5.  Back to cited text no. 16
    
17.
Muhammad-Lutfi AR, Zaraihah MR, Anuar-Ramdhan IM. Knowledge and practice of diabetic foot care in an in- patient setting at a tertiary medical center. Malays Orthop J 2014;8:22-6.  Back to cited text no. 17
    
18.
Shalom A, Friedman T, Wong L. Burns and diabetes. Ann Burns Fire Disasters 2005;18:31-3.  Back to cited text no. 18
    
19.
Sutariya P, Kharadi A. Knowledge and practice of foot care among the patients of diabetic foot: A hospital based cross-sectional study. Int Surg J 2016;3:1850-5.  Back to cited text no. 19
    
20.
Desalu OO, Salawu FK, Jimoh AK, Adekoya AO, Busari OA, Olokoba AB. Diabetic foot care: Self reported knowledge and practice among patients attending three tertiary hospital in Nigeria. Ghana Med J 2011;45:60-5.  Back to cited text no. 20
    
21.
Abdulghani HM, AlRajeh AS, AlSalman BH, AlTurki LS, AlNajashi NS, Irshad M, et al. Prevalence of diabetic comorbidities and knowledge and practices of foot care among diabetic patients: A cross-sectional study. Diabetes Metab Syndr Obes 2018;11:417-25.  Back to cited text no. 21
    
22.
Saber HJ, Daoud AS. Knowledge and practice about the foot care and the prevalence of the neuropathy among a sample of type 2 diabetic patients in Erbil, Iraq. J Family Med Prim Care 2018;7:967-74.  Back to cited text no. 22
[PUBMED]  [Full text]  
23.
Al-Asmary AS, Sama Mostafa OA, Al-khaldi YM, Mostafa OA. Diabetic patients' knowledge and practice regarding prevention of diabetic foot. Med J Cairo Univ 2013;81:197-205.  Back to cited text no. 23
    
24.
Chellan G, Srikumar S, Varma AK, Mangalanandan TS, Sundaram KR, Jayakumar RV, et al. Foot care practice-The key to prevent diabetic foot ulcers in India. Foot (Edinb) 2012;22:298-302.  Back to cited text no. 24
    
25.
Ramirez-Perdomo C, Perdomo-Romeroa A, Rodríguez-Véleza M. Knowledge and practices for the prevention of the diabetic foot. Rev Gaucha Enferm 2019;40:1-7.  Back to cited text no. 25
    
26.
Natalia de Sá P, Moura JR, de Melo Júnior EB, de Almeida PC, de Macêdo SF, da Silva AR. Knowledge, attitudes and practices for the prevention of diabetic foot. Rev Gaucha Enferm 2014;35:36-42. [in Por].  Back to cited text no. 26
    
27.
Pourkazemi A, Ghanbari A, Khojamli M, Balo H, Hemmati H, Jafaryparvar Z, et al. Diabetic foot care: Knowledge and practice. BMC Endocr Disord 2020;20:40.  Back to cited text no. 27
    
28.
Li R, Yuan L, Guo XH, Lou QQ, Zhao F, Shen L, et al. The current status of foot self-care knowledge, behaviours, and analysis of influencing factors in patients with type 2 diabetes mellitus in China. Int J Nurs Sci 2014;1:266-71.  Back to cited text no. 28
    
29.
Gökdeniz D, Akgün Şahin Z. Evaluation of knowledge levels about diabetes foot care and self-care activities in diabetic individuals. Int J Low Extrem Wounds 2022;21:65-74.  Back to cited text no. 29
    
30.
Yılmaz Karadağ F, Saltoğlu N, Ak Ö, Çınar Aydın G, Şenbayrak S, Erol S, et al. Foot self-care in diabetes mellitus: Evaluation of patient awareness. Prim Care Diabetes 2019;13:515-20.  Back to cited text no. 30
    
31.
Abu-Qamar MZ. Knowledge and practice of foot self-care among Jordanians with diabetes: An interview-based survey study. J Wound Care 2014;23:247-50, 252-4.  Back to cited text no. 31
    
32.
Chiwanga FS, Njelekela MA. Diabetic foot: Prevalence, knowledge, and foot self-care practices among diabetic patients in Dar es Salaam, Tanzania-A cross-sectional study. J Foot Ankle Res 2015;8:20.  Back to cited text no. 32
    
33.
Sari Y, Upoyo AS, Isworo A, Taufik A, Sumeru A, Anandari D, et al. Foot self-care behavior and its predictors in diabetic patients in Indonesia. BMC Res Notes 2020;13:38.  Back to cited text no. 33
    


    Figures

  [Figure 1], [Figure 2], [Figure 3]
 
 
    Tables

  [Table 1], [Table 2], [Table 3], [Table 4], [Table 5]



 

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