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A comprehensive review of identification methods for pathogenic yeasts: Challenges and approaches
Hamid Morovati1, Mohammad Kord2, Kazem Ahmadikia2, Saba Eslami3, Masoumeh Hemmatzadeh4, Kian M Kurdestani5, Mohammadreza Khademi6, Sima Darabian7
1 Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
2 Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
3 Central Research Laboratory, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
4 Department of Mycology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
5 Department of Microbiology, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran
6 Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran
7 Department of Medical Parasitology and Mycology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
Correspondence Address:
Dr. Sima Darabian
Department of Medical Parasitology and Mycology, Zanjan University of Medical Sciences, Karmandan Town, Mahdavi Blvd, School of Medicine, Zanjan - 4513956111
Iran
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/abr.abr_375_22
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Given the increasing incidence of yeast infections and the presence of drug-resistant isolates, accurate identification of the pathogenic yeasts is essential for the management of yeast infections. In this review, we tried to introduce the routine and novel techniques applied for yeast identification. Laboratory identification methods of pathogenic yeast are classified into three categories; I. conventional methods, including microscopical and culture-base methods II. biochemical/physiological-processes methods III. molecular methods. While conventional and biochemical methods require more precautions and are not specific in some cases, molecular diagnostic methods are the optimum tools for diagnosing pathogenic yeasts in a short time with high accuracy and specificity, and having various methods that cover different purposes, and affordable costs for researchers. Nucleotide sequencing is a reference or gold standard for identifying pathogenic yeasts. Since it is an expensive method, it is not widely used in developing countries. However, novel identification techniques are constantly updated, and we recommend further studies in this field. The results of this study will guide researchers in finding more accurate diagnostic method(s) for their studies in a short period of time.
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