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Brief Report:
Phospholipase activity of
Candida
species isolated from diabetic patients
Negar Amini, Rasoul Mohammadi
Adv Biomed Res
2023, 12:19 (27 January 2023)
DOI
:10.4103/abr.abr_87_22
Background:
Candidiasis is one of the prevalent fungal infections caused by the genus
Candida
. The clinical manifestation ranges from mucocutaneous colonization to disseminated and fatal infections such as candidemia. Diabetes mellitus is one of the significant predisposing factors for this fungal infection.
Candida
spp. may release many exoenzymes such as phospholipase to debilitate the immune system and facilitate adherence and invasion of the fungus to the host cells. The aim of the present study is evaluation of phospholipase activity of
Candida
species isolated from candidemia and gastroesophageal candidiasis (GEC) among diabetic patients.
Materials and Methods:
Eighty-three
Candida
isolates were evaluated for enzyme activity by phenotypic (the precipitation zone around the colonies) and molecular methods (detection of phospholipase genes using duplex polymerase chain reaction with specific primers).
Results:
Eight out of eighty-three clinical isolates (9.6%) were negative for phospholipase production. All phospholipase producers among candidemia and GEC isolates were categorized in high production group.
Conclusions:
Our findings revealed no differences in phospholipase activity among isolates obtained from different body sites (blood, oesophagus and stomach); however, non-
albicans Candida
species had less phospholipase activity.
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Brief Report:
Comparison of periodic in-person and remote visits via smartphone applications during COVID-19 pandemic in clinical follow-up of range of motion in patients with distal radius fracture
Amir Sabbaghzadeh, Sona Bonakdar, Maryam Khoshkholghsima, Melina Moshirpour, Mona Gorji, Morteza Gholipour
Adv Biomed Res
2022, 11:76 (27 September 2022)
DOI
:10.4103/abr.abr_47_21
Background:
Wrist function has a significant impact on quality of life, which is why restoring normal wrist movement after surgery is so important. Due to the COVID-19 pandemic, and the restrictions imposed on “face-to-face” visits, using smartphones has become more important in tracking patients. The main purpose of this study was to determine the accuracy of telemedicine in following up patients who had undergone distal radius fracture operation.
Materials and Methods:
From February to October 2020, 126 patients between 20 and 60 years old were randomly selected at our orthopedic trauma center. All patients were visited in person by an orthopedic surgeon in the morning (control group) and again all of them were visited online via smartphone in the evening by another orthopedic surgeon (case group). Both visits were done at regular intervals in the 2
nd
, 6
th
, and 12
th
weeks after surgery. Patients were evaluated for extremity function outcomes and joint range of motion.
Results:
The two groups were similar in terms of mean Patient-Rated Wrist Evaluation score and Disabilities of the Arm, Shoulder, and Hand score and did not show a statistically significant difference (
P
< 0.05). There was no significant difference in wrist range of motion measurements between the two groups (in-person visits and smartphone visits) during the follow-ups (
P
> 0.05).
Conclusion:
Changes in wrist range of motion after surgery can be assessed with high accuracy using smartphone applications and this method can be considered as a proper alternative to frequent in-person visits to evaluate postsurgical wrist condition.
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Brief Report:
Assessment of metabolic risk factors and heart-healthy lifestyle in atherosclerotic coronary artery disease patients undergoing percutaneous coronary intervention after a 6-month follow-up
Seyed Mohammad Hashemi Jazi, Armina Radmanesh, Masoumeh Sadeghi, Asieh Mansouri
Adv Biomed Res
2022, 11:15 (28 February 2022)
DOI
:10.4103/abr.abr_206_20
Background:
Mortality due to acute coronary syndrome (ACS) has dramatically diminished because of performing life-saving interventions. This study aims to assess the metabolic risk factors and heart healthy lifestyle following the first episode of ACS under percutaneous coronary intervention (PCI) treatment after the 6-month follow-up.
Materials and Methods:
This is a longitudinal study conducted on 40 patients who underwent PCI because of the first episode of ACS. The patients' information including age, weight, abdominal circumference, smoking, functional capacity, patients' metabolic equivalent of task (METS), and laboratory tests including triglycerides (TGs), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), creatinine, fasting blood sugar (FBS), and hemoglobin A1C were recorded before discharge and reassessed after 6-month follow-up.
Results:
The patients were dominantly male (92.5%) with an average age of 56.8 ± 7.11 years. Physical activity and functional capacity (METS) significantly improved within 6 months (
P
= 0.019). BMI significantly improved; however, although the abdominal circumference decreased, it was not significant (
P
= 0.28). The number of smokers (
P
= 0.12) and the daily number of smoked cigarettes (
P
= 0.37) nonsignificantly decreased within 6 months. However, HDL-C (
P
= 0.013) and LDL-C (
P
= 0.027) changes were not desirable. TG, FBS, and blood pressure did not statistically significant change (
P
> 0.05).
Conclusion:
Although BMI, physical activity, and METS remarkably improved, waist circumference decreased nonsignificantly and lipid profile got worse paradoxically. Although this population is limited for generalization, this study shows that we require further schedules to improve ACS secondary prevention practice in our community.
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Brief Report:
Evaluation of intra-articular corticosteroid injections in patients with sacroiliac pain
Ali Andalib, Mohammadreza Etemadifar, Mohammad Ansari Bardei
Adv Biomed Res
2022, 11:13 (28 February 2022)
DOI
:10.4103/abr.abr_100_20
Background:
Sacroiliac pain is one of the causes of low back pain, representing with discomfort and tenderness in the sacroiliac joint. Interventional procedures might be beneficial in cases unresponsive to medical treatments. Here, we aimed to investigate the effects of intra-articular corticosteroid injections in patients with sacroiliac pain.
Materials and Methods:
This is a clinical trial performed in 2017–2018 in Kashani Hospital, Isfahan, on patients with low back pain and formerly diagnosed with sacroiliac pain. Patients were recruited based on inclusion and exclusion criteria. Corticosteroid injections were performed for patients. Patients were visited within 2 weeks, 4 weeks, and 6 months after interventions, and pain and disabilities of patients were assessed using the numerical rating scale (NRS) and Oswestry Disability Questionnaire.
Results:
A total number of 27 patients entered our clinical trial. We showed that the mean NRS score among patients before interventions was 8.01 ± 0.96. Assessments of disability score also indicated that the mean disability scores was 41.48 ± 7.48. Our data also indicated that there was a significant reducing trend in both NRS and disability score after interventions (
P
< 0.001 for both items).
Conclusion:
Intra-articular steroid injection is associated with significantly reduced pain and disability in patients with sacroiliac pain. Previous studies evaluated variable methods and reported that this method has high values and significant advantages compared with other techniques which were in line with our results.
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Brief Report:
Keyes triad in type 2 diabetes mellitus: A microbiological study
Jochima Eudora Cota, Anita Dhupar, Anita Spadigam, Karla Carvalho
Adv Biomed Res
2021, 10:36 (26 November 2021)
DOI
:10.4103/abr.abr_160_20
Background:
With the increasing prevalence of type 2 diabetes mellitus (DM), there is a need to assess if the elevated salivary glucose levels provide an environment conducive to the growth of cariogenic microorganisms specifically
Streptocooccus mutans
and
Lactobacillus acidophilus.
Materials and Methods:
Forty-five patients were divided into three groups consisting of patients with type 2 DM with caries, patients with type 2 DM without caries and age-matched healthy nondiabetic individuals (control). Saliva samples were subjected to semiautomatic salivary glucose estimation by the glucose oxidase-peroxidase method, using the Tulip glucose estimation kit. Swabs were immediately inoculated onto Mitis Salivarius Bacitracin agar and Man Rogosa Sharpe agar.
Results:
In Group A, statistically significant positive correlation was found between
S. mutans
and salivary glucose (
r
= 0.858) as well as
L. acidophilus
and salivary glucose (
r
= 0.853). In Group B, a statistically significant positive correlation was found only between
S. mutans
and salivary glucose (
r
= 0.705) and not between
L. acidophilus
and salivary glucose (
r
= 0.387). The control group did not show a statistically significant correlation.
Conclusion:
It is established that salivary glucose levels reflect the diabetic state of an individual. The salivary glucose level predicted a 1.7 times higher caries susceptibility in a diabetic, as shown by results in this study. Salivary glucose causes an increase in the cariogenic load in diabetic patients, thus warranting a modification of the Keyes triad.
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Brief Report:
The prevalence of hepatitis B virus markers among students of Shiraz University of Medical Sciences
Razieh Dowran, Mahyar Malekzadeh, Tayyar Nourollahi, Bahador Sarkari, Jamal Sarvari
Adv Biomed Res
2021, 10:7 (26 February 2021)
DOI
:10.4103/abr.abr_173_20
Background:
Protection against hepatitis B virus (HBV) is based on the presence of antibodies against hepatitis B surface antigen (HBsAg). Vaccination of newborns is the most effective means of prevention. This study aimed to evaluate the frequency of anti-HBs antibody (anti-HBsAb), anti-HB core Ab (anti-HBcAb), HBsAg, and HBV DNA among university students in Fars province, Southern Iran.
Materials and Methods:
In this cross-sectional study, 272 students of Shiraz University of Medical Sciences, were enrolled. Venous blood (5 mL) was collected from each participant and centrifuged; the sera were stored at −20°C until use. Anti-HBsAb, Anti-HBcAb, and HBsAg were measured using a commercial enzyme-linked immunosorbent assay kit. HBV DNA load was also measured by a real-time polymerase chain reaction.
Results:
The mean age of the participants was 19 ± 1 years. There were 171 (62.9%) females and 101 (37.1%) males. Anti-HBsAb at a protective level (>10 mIU/mL) were detected in the sera of 104 (38.5%) of the cases. Of the anti-HBsAb seropositive participants, 82 were female and 22 were male; the difference between the gender and seropositivity to anti-HBsAb was statistically significant (
P
= 0.001, odds ratio: 3.3, 95% confidence interval = 1.89–5.79). Anti-HBcAb was detected in only one participant that was negative for both HBsAg and HBV DNA.
Conclusion:
Findings of the current study show that more than half of the students do not have a protective level of anti-HBsAb and might be susceptible to HBV infection, indicating the necessity of checking the level of anti-HBsAb as well as a booster dose in high-risk groups.
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Brief Report:
Outcomes of Implementation of Sacral Nerve Stimulation in Incontinent Patients in Shiraz
Alimohammad Bananzadeh, Seyed Vahid Hosseini, Ahmad Izadpanah, Alireza Izadi, Hajar Khazraei, Mozhdeh Zamani, Faranak Bahrami
Adv Biomed Res
2019, 8:21 (20 March 2019)
DOI
:10.4103/2277-9175.254624
PMID
:31016179
Background:
Fecal incontinence is a common disorder in old age; however, it may not threaten life, but it can cause morbidity and many problems. Sacral nerve stimulation (SNS) is a minimally invasive surgical procedure performed by chronic electrical stimulation of the nerves in the sacral plexus through a lead implanted at the S3 foramen. This study aimed to evaluate the outcomes of SNS in Shiraz.
Materials and Methods:
Data from patients who underwent implantation of an SNS device from 2012 to 2018 were reviewed in Shiraz. Thirty patients who had incontinence were evaluated by a committee. Pre- and postoperative assessments of the severity of incontinence were performed using Wexner Incontinence Score. Statistical analysis was performed using paired
t
-test.
Results:
Twenty-seven patients proceeded to insertion in the temporary SNS, and of these, 16 were elected to have a permanent SNS. Finally, seven patients were satisfied with their treatment. There was a significant reduction in the pre- and post-SNS Wexner Incontinence Scores from a median of 15–10, respectively (
P
< 0.05).
Conclusion:
In our study, 16 patients underwent SNS protocol, and 43.7% of them showed a good response and recovered. It is recommended as a method for the treatment of fecal incontinence. Permanent SNS is effective, showing a significant improvement in fecal incontinence scores.
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Brief Report:
Assessment of the Early and Late Complication after Thyroidectomy
Esmaeil Chahardahmasumi, Rezvan Salehidoost, Massoud Amini, Ashraf Aminorroaya, Hassan Rezvanian, Ali Kachooei, Bijan Iraj, Masoud Nazem, Mohsen Kolahdoozan
Adv Biomed Res
2019, 8:14 (27 February 2019)
DOI
:10.4103/2277-9175.253115
PMID
:30993084
Background:
The complications in thyroid surgery have been reported variable in literature. The aim of this study was to evaluate the early and late (3 months after surgery) complication rates of thyroidectomy in a cohort of patients undergoing thyroid surgery at two hospitals of Isfahan University of Medical Science, Iran.
Materials and Methods:
This study included 204 patients who candidates for thyroidectomy presenting at Medical Educational Centers of Al-Zahra and Kashani hospitals in Isfahan between March 2016 and March 2017. Clinical data are collected for all patients by continuous enrollment. The patients examined before and after thyroid surgery and the findings were recorded.
Results:
The highest prevalence of thyroidectomy was in women (81.9%). The most frequent thyroid surgery was total thyroidectomy and the most common indication for thyroid surgery was suspicious fine-needle aspiration for thyroid malignancy. Hypocalcemia was the most common complication with a frequency of 54.4%. The odds ratios for early complications were 2.375 and 2.542 for intermediate- and low-volume surgeons, respectively, compared to high-volume surgeons.
Conclusions:
According to the results of this study, the high level of surgeon's skill is effective to reduce the likelihood of late and early complications; furthermore, the chance of late complications increases with age.
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Brief Report:
Effect of Incise Drape on Contamination Rate of Surgical Wound during Surgical Procedures of Lumbar Spine
Mohammadreza Zarei, Homayoun Tabesh, Hossein Fazeli, Akram Aarabi
Adv Biomed Res
2019, 8:8 (31 January 2019)
DOI
:10.4103/2277-9175.251213
PMID
:30820429
Background:
The aim of this study was to investigate the effect of the incise drape (ID) on surgical wound bacterial contamination during lumbar spine surgical procedures in treatment group (with ID) and control group (without ID).
Materials and Methods:
The present study was conducted on 88 patients who were a candidate for lumbar spine surgery. The patients were randomly assigned to one of the two groups, treatment and control. The ID was only used in the treatment group. The surgical wound sampling for bacterial culture was done in two steps, immediately after surgical incision (IASI) and immediately prior to the surgical wound closure (IPSWC). The samples were then sent to the laboratory.
Results:
The mean total bacterial count of the surgical wound in the stage IASI was not significantly different between treatment and control groups (0.09 vs. 0.02,
P
= 0.31). However, this means in the stage IPSWC in treatment group was significantly more than the control group (18.6 vs. 0.41,
P
= 0.04). The frequency distribution of
Staphylococcus aureus
(25% vs. 3%,
P
= 0.02) and
Staphylococcus epidermidis
(36.4% vs. 9.1%,
P
= 0.002) was significantly higher in the treatment group compared with control group in the stage IPSWC.
Conclusion:
The results suggest that the use of ID is unable to reduce surgical wound bacterial contamination in clean lumbar spine surgery. Therefore, based on the results obtained in our study, the application of ID is not recommended as an essential action for the prevention of surgical wound contamination.
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Brief Report:
Early Versus Delayed (Traditional) Postoperative Oral Feeding in Patients Undergoing Colorectal Anastomosis
Behzad Nematihonar, Sohrab Salimi, Vahid Noorian, Majid Samsami
Adv Biomed Res
2018, 7:30 (16 February 2018)
DOI
:10.4103/abr.abr_290_16
PMID
:29531928
Background:
A period of starvation after colorectal anastomosis to permit for resolution of the clinical evidence of ileus has been an unchallenged surgical dogma until recent years. We intended to determine the safety and feasibility of an unconventional postoperative oral intake protocol in patients experiencing colorectal anastomosis.
Materials and Methods:
Between 2013 and 2015, sixty consecutive patients underwent colorectal anastomosis and they were randomized into two groups. The early feeding group began fluids on the first postoperative day while the regular feeding group was managed in the traditional way - nothing by mouth until the complete resolution of ileus.
Results:
The majority of patients (93%) tolerated the early feeding. The times to first passage of flatus (2.66 ± 0.71 days vs. 3.9 ± 0.071 days) and stool (3.9 ± 0.92 days vs. 5.4 ± 0.77 days) were significantly quicker in early feeding group. Hospital stay was also significantly shorter in the early feeding group (4 ± 0.64 days vs. 6.1 ± 0.84 days). Anastomosis leakage and abscess formation were not seen in early feeding group. The patient's satisfaction (visual analog scale) in the early feeding group was higher than delayed feeding group (8.56 ± 1.16 vs. 7.06 ± 1.59,
P
< 0.001).
Conclusions:
Early oral feeding after colorectal surgeries is safe and tolerated by the majority of patients.
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Brief Report:
Comparison of Nasal Mucociliary Function before and after Septorhinoplasty Surgery Using Saccharin Test
Mostafa Hashemi, Ali Reza Dadgostar, Nezamoddin Berjis
Adv Biomed Res
2017, 6:160 (26 December 2017)
DOI
:10.4103/abr.abr_171_15
PMID
:29387671
Background:
This study was carried out to compare mucociliary function (MCF) of nasal cavity before and after septorhinoplasty surgery by saccharin test.
Materials and Methods:
This study was carried out on 70 patients who needed septorhinoplasty surgery in Kashani and Alzahra Hospital during 2013–2014 to measure MCF. Saccharin test was performed on the patients before surgery and on three more intervals 1 day, 1 week, and on month after ward and the readings were recorded. Saccharin test is performed by placing saccharin granules on the anterior part of inferior turbinate and the time the patient feels the sweet taste is recorded the normal range is below 30 min.
Results:
Mean of test for each time interval was obtained and comparison of measurements was made reading were mean of saccharin test before surgery (7.74 ± 1.65 min) the day after surgery (7.75 ± 1.7 min), on week after surgery (15.04 ± 3.4), and 1 month after surgery (8.49 ± 2.08 min). The readings before surgery did not have statistically significant difference with the readings 1 day after surgery
P
= 0.99, but comparison of test results at other time intervals were significant (
P
< 0.001). A relative increase in saccharin test time was seen in a period of 1 month after surgery the rise was more noticeable in the 1
st
week compared to 1 month after surgery.
Conclusion:
This study in the period after septorhinoplasty surgery MCF undergoes slight impairment that gradually progresses to normal values during 1 month after surgery.
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Brief Report:
An Evaluation of Ultrasound Features of Breast Fibroadenoma
Alireza Namazi, Atoosa Adibi, Mahshid Haghighi, Morteza Hashemi
Adv Biomed Res
2017, 6:153 (30 November 2017)
DOI
:10.4103/2277-9175.219418
PMID
:29285483
Background:
Breast cancer is among the most common cancers in the world. Ultrasound evaluations of breast have come into attention as an alternative route. Ultrasound features of benign lesions such as fibroadenoma can be overlapping with those in a malignant tumor. Here, we assessed the reports of breast ultrasound in patients with pathologic diagnosis of fibroadenoma.
Materials and Methods:
We conducted a cross-sectional study and enrolled female patients with confirmed histologic diagnosis of fibroadenoma. Ultrasound studies were performed on the participants to see which sonographic patterns are more frequent in such lesions.
Results:
In 92 patients with 40.4 ± 9.2 years of age, all participants were classified as stage 4 on Breast Imaging-Reporting and Data System scale. The mean ± standard deviation of size for the lesions was 167.4 ± 101.4 mm
2
. Upper outer quadrants in the breasts had the most number of lesions. Almost lesions were round with only 2.2% were oval. When assessed for the margin definition, 57.8% were circumscribed. Noncircumscribed masses were reported in 21.7%. About 91.3% of cases were hypoechoic in the ultrasound evaluation. Lobulated masses were in 28.3% of the cases. 8.7% of the masses were spongy whereas 9.8% and 2.2% of them had calcification and heterogenic appearance, respectively.
Conclusion:
The most frequent features include a hypoechoic mass with a circumscribed border; however, complex presentations that overlap malignant masses are also detectable including noncircumscribed margin, lobulation, presence of a posterior shadow, heterogenicity, and micro calcification.
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Brief Report:
Comparing the Frequency of Endometritis in Unexplained Infertility and Anovulatory Infertility
Ataollah Ghahiri, Bahare Malekzadeh, Hatav Ghassemi Tehrani
Adv Biomed Res
2017, 6:151 (30 November 2017)
DOI
:10.4103/2277-9175.219416
PMID
:29285481
Background:
Acute pelvic inflammatory disease (PID) is a common reason for infertility. This study aimed to evaluate the frequency distribution of endometritis in women with unexplained infertility and comparison with frequency distribution of endometritis in anovulatory infertility to identify the importance of endometritis due to subacute PID evaluation in the case of infertility.
Materials and Methods:
This case–control study was done on 100 women with unexplained infertility and ovulatory infertility who referred to Shahid Beheshti clinic in 2013 in Isfahan, Iran. They were divided into two groups of unexplained infertility and anovulatory infertility. Endometrial samples were given from all the patients by Pipelle biopsy under sterile conditions, and then prepared samples were sent to the pathology laboratory to evaluate the existence of plasma cells by a pathologist to diagnose endometritis.
Results:
Frequency distribution of acute PID history among the patients in both groups showed a significant difference (
P
< 0.05). Prevalence of endometritis in unexplained infertility group was 34% and in anovulatory group was 21% (
P
< 0.05). Prevalence of vaginitis was 46% in unexplained group and 40% in anovulatory group (
P
< 0.05), and prevalence of PID was 4% in unexplained infertility group and 0% in anovulatory infertility group.
Conclusion:
The prevalence of endometritis and vaginitis was more in the unexplained infertility group rather than the anovulatory infertility group that may reveal the importance of endometritis evaluation in the cases of unexplained infertility.
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Brief Report:
Limited Approach in Endoscopic Dacryocystorhinostomy of Pediatrics
Seyyed Mostafa Hashemi, Afrooz Eshaghian
Adv Biomed Res
2017, 6:141 (10 November 2017)
DOI
:10.4103/abr.abr_375_14
PMID
:29279839
Background:
Limited spatial nasal cavity in children, make pediatric dacryocystorhinostomy (DCR) a difficult surgical procedure. We apply a limited approach to pediatric DCR and follow them for their consequences.
Materials and Methods:
An experimental study was done in pediatric DCR with limited approach (age < 14-year-old). After written consent, with general anesthesia, with nasal endoscopic surgery, lacrimal bone is exposed and extruded. In contrast with routine procedure, ascending process of maxillary sinus reserve; and marsupialization and wide exposure to lacrimal sac was done only by lacrimal bone defect; and cannulation preserve with temporary silicone tube.
Results:
Between 2006 and 2012, 16 pediatric DCR was done by a unique surgeon in 2 otorhinolaryngologic centers. Before surgery 14 (87.5%) had epiphora, 3 (18.8%) had eye discharge, and 3 (18.8%) had eye sticky eye. Two (12.5%) had history of facial trauma, and 10 (62.5%) had congenital nasolacrimal duct insufficiency. Five (31.3%) had history of dacryocystitis. Patients were followed for 17 ± 9 months. Silicone tube stayed for 4 ± 2.5 months. We could follow 7 patients and minimal improvement or need to revision surgery considered as technical failure. After surgery, 3 patients had no epiphora with complete improvement; 2 had very good improvement with confidence of the patients and parents; 2 cases had unsuccessful surgery in our patients, who needs to another surgery. One of them had several probing and surgery before our endoscopic DCR.
Conclusions:
Limited approach in endoscopic DCR of pediatrics can be done in noncomplicated patients, with minimal manipulation, more confidence, and acceptable results.
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Brief Report:
Optimal DNA Isolation Method for Detection of Nontuberculous Mycobacteria by Polymerase Chain Reaction
Samira Mohammadi, Bahram Nasr Esfahani, Sharareh Moghim, Hossein Mirhendi, Fatemeh Riyahi Zaniani, Hajieh Ghasemian Safaei, Hossein Fazeli, Mahshid Salehi
Adv Biomed Res
2017, 6:133 (25 October 2017)
DOI
:10.4103/2277-9175.217216
PMID
:29279831
Background:
Nontuberculous mycobacteria (NTM) are a group of opportunistic pathogens and these are widely dispersed in water and soil resources. Identification of mycobacteria isolates by conventional methods including biochemical tests, growth rates, colony pigmentation, and presence of acid-fast bacilli is widely used, but these methods are time-consuming, labor-intensive, and may sometimes remain inconclusive.
Materials and Methods:
The DNA was extracted from NTM cultures using CTAB, Chelex, Chelex + Nonidet P-40, FTA
®
Elute card, and boiling The quantity and quality of the DNA extracted via these methods were determined using UV-photometer at 260 and 280 nm, and polymerase chain reaction (PCR) amplification of the heat-shock protein 65 gene with serially diluted DNA samples.
Results:
The CTAB method showed more positive results at 1:10–1:100,000 at which the DNA amount was substantial. With the Chelex method of DNA extraction, PCR amplification was detected at 1:10 and 1:1000 dilutions.
Conclusions:
According to the electrophoresis results, the CTAB and Chelex DNA extraction methods were more successful in comparison with the others as regard producing suitable concentrations of DNA with the minimum use of PCR inhibitor.
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Brief Report:
Rapid Detection of Streptomycin-Resistant
Mycobacterium tuberculosis
by
rpsL
-Restriction Fragment Length Polymorphism
Sediqe Karimi, Hossein Mirhendi, Fatemh Riyahi Zaniani, Soroor Erfani Manesh, Mahshd Salehi, Bahram Nasr Esfahani
Adv Biomed Res
2017, 6:126 (16 October 2017)
DOI
:10.4103/abr.abr_240_16
PMID
:29142889
Background:
Molecular methods for the detection of drug-resistant tuberculosis (DR-TB) are potentially more rapid than conventional culture-based drug susceptibility testing, facilitating the commencement of appropriate treatment for patients with DR-TB. The aim of this study was to evaluate and develop polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assays for the detection of mutations within
rpsL
, and for the determination of streptomycin (STR) resistance in
Mycobacterium tuberculosis
.
Materials and Methods:
Clinical specimens were collected from individuals with suspected TB referred to the TB Center of Isfahan' from which 205
M. tuberclosis
were isolated and identified by conventional phenotypic methods. The minimum inhibitory concentration of STR for all isolates was determined using the proportion method and 10 isolates were recognized as STR resistant
M. tuberculosis
. The effect of genetic alterations in the
rpsL
gene for these resistant isolates were investigated by PCR-RFLP method.
Results:
Three (30%) isolates showed point mutation at codon 43 by RLFP analysis.
Conclusion:
Our results suggest that RFLP analysis of the
rpsL
gene is useful for the rapid prediction of STR resistant strains of
M. tuberculosis
.
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Brief Report:
The Immune Response of Vaccination Against Hepatitis B virus in Iranian Patients Undergoing Chemotherapy
Mohsen Meidani, Farzin Khorvash, Simin Hemati, Farzaneh Ashrafi, Behrouz Ataei, Dana Daneshmand
Adv Biomed Res
2017, 6:88 (25 July 2017)
DOI
:10.4103/abr.abr_330_13
PMID
:28828339
Introduction:
Hepatitis B virus (HBV) infection and its complications are major public health problems. As it is hard to treat and control the chronic state, control of disease depends on the prevention especially by vaccination. There is an impaired immune response to vaccinations including HBV in patients with some malignancies. The aim of this study is to assess the response rate of patients undergoing chemotherapy to HBV vaccination.
Materials and Methods:
All patients from two hematology/oncology clinics in Isfahan, Iran with the history of at least 1 month chemotherapy who had the inclusion criteria were enrolled in a case control study. Also a sex- and age-matched control group from healthy population was selected. They were vaccinated in a schedule of 0, 1, and 6 months and were examined for antibody titers 1 month after the last dose. The titers more than 10 mIU/ml were determined as positive response to vaccination.
Results:
In this study, 50 patients and 50 healthy subjects were enrolled. The two groups were age and sex matched (
P
> 0.05). Frequency of negative responses to HBV vaccination in case and control groups were 9 (18%) and 1 (2%), respectively (OR = 10.75, CI = 1.30–88.47,
P
= 0.027). Of 50 patients, 54%, 12%, 22%, and 12% had breast cancer, lymphoma, gastrointestinal, and genitourinary cancers, respectively, and frequency of negative responses were 3 (11%), 1 (16%), 4 (36.4%), and 1 (16%), respectively (
P
= 0.167).
Conclusion:
According to our results, malignancy and chemotherapy will have an important effect on the immune system and cause negative response to HBV vaccination. Our results revealed the importance of passive immunity and screening for HBV infection in patients undergoing chemotherapy. Also more studies for better vaccination schedules in this group of patients are recommended.
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Brief Report:
Nosocomial Infections in Intensive Care Unit: Pattern of Antibiotic-resistance in Iranian Community
Bahram Nasr Esfahani, Rozita Basiri, Seyed Mohammad Mahdy Mirhosseini, Sharareh Moghim, Shahaboddin Dolatkhah
Adv Biomed Res
2017, 6:54 (2 May 2017)
DOI
:10.4103/2277-9175.205527
PMID
:28553627
Background:
Bacterial infections are responsible for great number of mortality in Intensive Care Unit (ICU). Knowledge about prevalence of bacterial infections and their antibiotic-resistance pattern would be a great step for their treatment and management.
Materials and Methods:
Data about nosocomial infections in ICUs of Alzahra Hospital (referral hospital in Isfahan, center of Iran) were gathered during the years 2007–2010. A questionnaire was fulfilled for any specific patient with nosocomial infection containing demographic data of patient and also characteristics of the infection.
Results:
Out of all patients, 707 individuals (65.6%) were male and 370 (34.4%) were female. Our data revealed that
Pseudomonas aeruginosa
(13.9%),
Klebsiella
(11%), and
Escherichia coli
(6.4%) were the most prevalent bacterial infections. The most common sites of nosocomial infections in the ICU were respiratory system (399 cases, 37%), urinary system (230 cases, 21.4%), and blood (102 cases, 9.5%). The antibiotic-resistance of each bacteria in ICU ward was assessed and data were categorized in a table. There were less documentary about bacterial cultures in the year 2007 when compared with the next years.
Conclusion:
We found some differences (such as bacterial prevalence in ICU wards which caused nosocomial infections) in our local prevalence of nosocomial infections and also in their resistance pattern compared to other centers. Knowing about our data will help physicians to administer the most suitable antibiotics for treatment of nosocomial infections in our area.
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Brief Report:
The Prevalence of Osmophobia in Migranous and Episodic Tension Type Headaches
Ahmad Chitsaz, Abbas Ghorbani, Masoumeh Dashti, Mohsen Khosravi, Mohammadreza Kianmehr
Adv Biomed Res
2017, 6:44 (17 April 2017)
DOI
:10.4103/2277-9175.204587
PMID
:28503499
Background:
Migraines are a neurological disease, of which the most common symptom is an intense and disabling episodic headache. Many persons experience sensory hyper excitability manifested by photophobia, phonophobia and osmophobia. This study was planned to investigate the prevalence of osmophobia in migranous and episodic tension type headache (ETTH).
Materials and Methods:
A semi-structured questionnaire was administered to all patients to evaluate the eventual presence of osmophobia during a headache attack and different characteristics of osmophobia were determined.
Results:
Osmophobia reported in 84% with migranous headache with aura, 74% of migranous patients without aura and in 43.3% of those with ETTH.
In 50% of patients, osmophobia was present in all of their headache attacks, 11.7% had osmophobia in more than half of their attacks (from 10 attacks they reported osmophobia in 5-9 ones) and others had this sign in less than half of their attacks (from 10 attacks they reported osmophobia in less than 5 ones). Most frequently the offending odors were scents (88%), foods (54.2%) and cigarette smoke (62.5%). Osmophobia starts 30 min before the headache starts in 22.7% of patients.
Conclusion:
Osmophobia appears structurally integrated into the migraine history of the patient; however, for differential diagnosis with ETTH, other criteria are necessary.
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Brief Report:
Evaluation of Energy Balance on Human Telomerase Reverse Transcriptase (hTERT) Alternative Splicing by Semi-quantitative RT-PCR in Human Umbilical Vein Endothelial Cells
Mohaddeseh Behjati, Mohammad Hashemi, Mohammad Kazemi, Mansoor Salehi, Shaghayegh Haghjooy Javanmard
Adv Biomed Res
2017, 6:43 (17 April 2017)
DOI
:10.4103/2277-9175.204591
PMID
:28503498
Background:
Decreased high-energy phosphate level is involved in endothelial cell injury and dysfunction. Reduced telomerase activity in endothelial cells in parallel with reduced energy levels might be due to altered direction of alternative splicing machine as a complication of depleted energy during the process of atherosclerosis.
Materials and Methods:
Isolated human umbilical vein endothelial cells (HUVECs) were treated for 24 hours by oligomycine (OM) and 2-deoxy glucose (2-DG). After 24 hours, the effect of energy depletion on telomerase splicing pattern was evaluated using RT-PCR. Indeed, in both treated and untargeted cells, nitric oxide (NO) and von Willebrand factor (vWF) were measured.
Results:
ATP was depleted in treated cells by 43.9% compared with control group. We observed a slight decrease in NO levels (
P
= 0.09) and vWF (
P
= 0.395) in the setting of 49.36% ATP depletion. In both groups, no telomerase gene expression was seen. Telomerase and housekeeping gene expression were found in positive control group (colon cancer tissue) and sample tissue.
Conclusions:
The absence of telomerase gene expression in HUVECs might be due to the mortality of these cells or the low level of telomerase gene expression in these cells under normal circumstances.
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Brief Report:
The Investigation of Insulin Resistance in Two Groups of Epileptic Patients Treated with Sodium Valproate and Carbamazepine
Mohammad Reza Najafi, Bahareh Bazooyar, Mohammad Zare, Mohammad Reza Aghaghazvini, Behnaz Ansari, Ali Rajaei, Masoumeh Dashti
Adv Biomed Res
2017, 6:25 (7 March 2017)
DOI
:10.4103/2277-9175.201689
PMID
:28401072
Background:
Valproic acid (VPA) is a widely used broad-spectrum antiepileptic drug for therapy of generalized and focal epilepsies. Cross-sectional studies have suggested that valproate treatment may be associated with hyperinsulinemia. We decided to investigate hyperinsulinemia as a health-threatening side effect of VPA in Iranian epileptic patients.
Materials and Methods:
Body mass index (BMI), lipid profile, fasting serum insulin, fasting blood glucose (FBS), and homeostatic model assessment-insulin resistance (HOMA-IR) were measured in 30 VPA-treated epileptic patients and 30 controls (CBZ-treated). The Chi-square test,
t
-test, and Pearson correlation test were used.
Results:
BMI was higher in VPA group than in control group (25.7 ± 3.5 > 21.7 ± 4.1) (0.000 < 0.05). Prevalence of obesity was 16.6% in VPA group that was almost the same and even lower than general Iranian population. Serum triglyceride (TG) (150 ± 77.2) was higher than CBZ group (114 ± 35.2) (
P
= 0.023 < 0.05). However, serum high-density lipoprotein level was lower in VPA group than controls (45.2 ± 11.7 < 54.4 ± 13.9) (
P
= 0.008 < 0.05). Serum insulin, FBS, HOMA-IR, cholesterol, and low-density lipoprotein did not demonstrate statistically significant differences between the two groups (
P
> 0.05).
Conclusion:
Despite the majority of previous studies that are against VPA and according to our study, VPA could be prescribed safely and it may not cause IR and its complications.
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Brief Report:
Wound Healing Effect of
Arnebia euchroma
gel on Excisional Wounds in Rats
Maryam Mohsenikia, Shahrzad Khakpour, Zahra Azizian, Soheil Ashkani-Esfahani, Seyyede Tahereh Razavipour, Pooya Toghiani
Adv Biomed Res
2017, 6:2 (31 January 2017)
DOI
:10.4103/2277-9175.199260
PMID
:28217647
Background:
Wound healing is a complex process leading to regeneration of damaged skin tissue.
Arnebia euchroma
(AE) have many effective activities such as anti-inflammatory, antimicrobial, antioxidative, and anti-tumoral effects. The extract of AE has positive effects on burn wounds. This study is designed to investigate the healing effects of AE on excisional wounds in rats.
Materials and Methods:
Thirty six rats with the age of 8 weeks divided into three groups. One group (E1) was treated with AE gel at a concentration of 10%. Control group (C1) received normal saline and the vehicle group (C2) was treated with carboxymethyl cellulose gel as the vehicle for 14 days. Stereological analysis was done to investigate the collagen bundle and hair follicale synthesis, vascularization, fibroblast proliferation. Pathological evaluation was also conducted.
Results:
In this study, pathological evaluation showed severe acute inflammation in C2 group, chronic and acute inflammation in C1 and also more wound contraction in E1 in comparison with other groups. There was a meaningful difference between E1 and C1 regarding fibroblast proliferation (
P
< 0.05).
Conclusion:
Results of this study revealed the healing effect of AE on excisional wounds and recommend its administration after further clinical investigations.
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Brief Report:
A randomized controlled trial comparing the effect of intravenous, subcutaneous, and intranasal fentanyl for pain management in patients undergoing cesarean section
Mitra Jabalameli, Reihanak Talakoub, Bita Abedi, Zahra Ghofrani
Adv Biomed Res
2016, 5:198 (27 December 2016)
DOI
:10.4103/2277-9175.190989
Background:
The objective of this study was to evaluate and compare the effects of three methods of using intravenous (IV), subcutaneous and intranasal (IN) fentanyl for pain management following general anesthesia in patients undergoing cesarean section.
Materials and Methods:
A prospective, randomized, single-blind clinical trial was done on 75 patients aged 20–40 years, American Society of Anesthesiology-1, who had a normal singleton pregnancy beyond 36 weeks of gestational age. Patients were randomized to receive 50 μg fentanyl intravenously (Group 1), subcutaneously (Group 2) or intranasally (Group 3) after closure of incision. The pain intensity, nausea, the systolic, and diastolic blood pressures were assessed.
Results:
All groups were equivalent for baseline characteristics. The average pain visual analog scale (VAS) score was less in the second group who received fentanyl subcutaneously at the time of recovery admission (6.8 ± 1.5) (
P
= 0.037) and after 3 h (6.36 ± 1.5) (
P
= 0.033) postoperatively. The mean VAS score of nausea and the mean systolic and diastolic blood pressures were not significantly different between three groups throughout the study (
P
> 0.05).
Conclusion:
subcutaneous fentanyl is an effective alternative to IV and IN route of administration for pain management.
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Brief Report:
Tangibility of platelet-rich fibrin matrix for nasolabial folds
Mehdi Rasti Ardakani, Hamed Parsa Moein, Mozhdeh Beiraghdar
Adv Biomed Res
2016, 5:197 (27 December 2016)
DOI
:10.4103/2277-9175.190987
Background:
Using autologous tissue is a simple process and ideal choice for augmentation of the soft facial tissue. In this regard, platelet-rich fibrin matrix (PRFM) is used for the correction of fine rhytids and nasolabial folds (NLFs) as well as facial augmentation. This study tries to evaluate the tangibility of PRFM for facial augmentation.
Materials and Methods:
In this study, 20 patients (20–45 years) were studied to examine the augmentation (4 patients were excluded). For each, 3cc of PRFM provided by using 35cc of autologous blood and injected through subdermal technique into NLFs. Thickness and volume of the tissue were measured before and 3 months after treatment by sonography. The collected data were analyzed through paired
t
-test and independent
t-
test by using SPSS version 19.0 (SPSS Inc., Chicago, IL, USA).
Results:
The study included 16 women, with average of 39.43 ± 6.84 years (age range, 20–45 years). The thickness and the volume of subcutaneous tissue in NLFs before and after treatment show no significant difference on the right side rather than on the left side (
P
> 0.05), but the thickness and the volume of the tissue on both sides after treatment shows significant increase rather than before treatment, so that this increase was significant statistically. This study shows that different right and left thickness was 0.54 ± 0.51 and 0.51 ± 0.38 (
P
≤ 0.001). Further, different right and left volume was 0.25 ± 0.20 and 0.26 ± 0.22, respectively (
P
< 0.001).
Conclusion:
According to the results, the increased tissue volume by PRFM still persists after 3 months of treatment and its retention has been effective on the healing process.
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Brief Report:
Investigation of changes in brain natriuretic peptide serum levels and its diagnostic value in patients with mild and moderate head trauma, in patients referred to emergency department of Alzahra Hospital, Isfahan, 2013-2014
Reza Azizkhani, Es'haq Keshavarz
Adv Biomed Res
2016, 5:191 (27 December 2016)
DOI
:10.4103/2277-9175.190983
Background:
Head trauma is one of the most common reasons for emergency department (ED) care. Over the past decade, initial management strategies in mild and moderate head trauma have become focused on selective computed tomography (CT) use based upon presence or absence of specific aspects of patient history and/or clinical examination which has received more attention following reports of increased cancer risk from CT scans. Recently changes in serum brain natriuretic peptide (BNP) levels following head trauma have been studied. We investigated the changes in serum levels of BNP in patients with mild and moderate head trauma, in whom the first brain CT scanning was normal.
Materials and Methods:
This study is a cross-sectional, descriptive research. It was performed in patients with mild and moderate head trauma. Forty-one patients with isolated mild and moderate traumatic brain injury (Glasgow Coma Scale = 9–15) were included. First brain CT scans were obtained during 2 h after ED arrival and the second one after 24 h. Plasma BNP levels were determined using a specific immunoassay system.
Results:
Twenty-three patients were in Group A (with normal first and second brain CT) and 18 patients in Group B (with normal first and abnormal second brain CT). With
P
= 0.001, serum BNP level = 9.04 was determined for differentiating two groups.
Conclusion:
We concluded that serum BNP level is higher in patients with mild and moderate head trauma with delayed pathologic changes in second brain CT relative to patients with mild and moderate head trauma and with normal delayed brain CT.
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Brief Report:
Trend and manifestations of falciparum malaria in a tertiary care hospital of India
Rama Prakasha Saya, Ganesh Kumar Saya, Goswami Debabrata
Adv Biomed Res
2016, 5:128 (29 July 2016)
DOI
:10.4103/2277-9175.187009
PMID
:27563638
Background:
The recent focus is on the increase in the burden of falciparum cases with a varied spectrum of presentation and outcome, especially in developing countries like India. This study was undertaken to analyze the trend and manifestations of falciparum malaria in a tertiary care hospital.
Materials and Methods:
This descriptive study was carried out at the Gauhati Government Medical College and Hospital from June 2006 to May 2007. The data were collected on demographic and time characteristics, clinical and laboratory findings, the outcome of disease and expressed in proportion or percentages.
Results:
Out of the 100 cases, around 2
nd
/3
rd
(63%) of cases were in the age group of 15–30 years and the mean age was found to be 29.51 years. About 66% of them were males. Clinical presentations included pain abdomen (42, 42%), nausea and vomiting (35, 35%), jaundice (34, 34%), oliguria (24, 24%), altered sensorium (24, 24%), breathing difficulty (10, 10%), and seizures (5, 5%). Number of cases and mortality were more with a peak in the month of May and September. Manifestations of severe falciparum malaria included hepatopathy (38%), renal failure (28%), shock (9%), acute respiratory distress syndrome (7%), hypoglycemia (3%), and severe anemia (1%). Eighty-two cases (82%) recovered and 18 cases (18%) expired.
Conclusion:
Falciparum malaria is more among younger adult age group and males. Complications and mortality are also more due to falciparum malaria.
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Brief Report:
The role of S-methylisothiourea hemisulfate as inducible nitric oxide synthase inhibitor against kidney iron deposition in iron overload rats
Maryam Maleki, Melika Samadi, Mehrangiz Khanmoradi, Mehdi Nematbakhsh, Ardeshir Talebi, Hamid Nasri
Adv Biomed Res
2016, 5:96 (30 May 2016)
DOI
:10.4103/2277-9175.183145
PMID
:27308268
Background:
Iron dextran is in common use to maintain iron stores. However, it is potentially toxic and may lead to iron deposition (ID) and impair functions of organs. Iron overload can regulate the expression of inducible nitric oxide synthase (iNOS) in some cells that has an important role in tissue destruction. S-methylisothiourea hemisulfate (SMT) is a direct inhibitor of iNOS, and this study was designed to investigate the effect of SMT against kidney ID in iron overload rats.
Materials and Methods:
24 Wistar rats (male and female) were randomly assigned to two groups. Iron overloading was performed by iron dextran 100 mg/kg/day every other day for 2 weeks. In addition, during the study, groups 1 and 2 received vehicle and SMT (10 mg/kg, ip), respectively. Finally, blood samples were obtained, and the kidneys were prepared for histopathological procedures.
Results:
SMT significantly reduced the serum levels of creatinine and blood urea nitrogen. However, SMT did not alter the serum levels of iron and nitrite, and the kidney tissue level of nitrite. Co-administration of SMT with iron dextran did not attenuate the ID in the kidney.
Conclusion:
SMT, as a specific iNOS inhibitor, could not protect the kidney from ID while it attenuated the serum levels of kidney function biomarkers.
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Brief Report:
Comparison of serological and molecular test for diagnosis of infectious mononucleosis
Hassan Salehi, Marziyeh Salehi, Rasoul Roghanian, Majid Bozari, Shirin Taleifard, Mohamad Mahdi Salehi, Maryam Salehi
Adv Biomed Res
2016, 5:95 (30 May 2016)
DOI
:10.4103/2277-9175.183144
PMID
:27308267
Background:
Epstein-Bar virus (EBV) is the main etiology of infectious mononucleosis (IM) syndrome that is characterized by fever, sore throat, and lymph adenopathy. Since, this virus could be associated with a number of malignancies, some hematologic disorders, and chronic fatigue syndrome, identification of IM is very important. The aim of study was to evaluate the specificity, as well as sensitivity of the two different methods that is, serology versus molecular diagnosis that are currently used for diagnosis of IM.
Materials and Methods:
In this study, during a period of 3.5 years, 100 suspected patients as case group and 100 healthy individuals as a control group were studied. Fifty samples in each group were tested by polymerase chain reaction (PCR) and all the samples including case group and control group were carried out by enzyme-linked immunosorbent assay (ELISA).
Results:
In 76% of patients and in 20% of the healthy individuals, samples were detected EBV DNA by PCR. On the other hand, 68.5% of the samples belong to the case group and 46% in the control group showed positivity by ELISA.
Conclusion:
By comparing the two methods, since PCR is very expensive and time consuming, and the percentages of difference ranges are narrow, ELISA could be applied as a first, easiest, and preliminary diagnostic test for IM. In addition, this test could be applied in various phases of the disease with a higher sensitivity comparing to PCR. Although PCR is routinely used for diagnosis of various infectious agents, it is considered as an expensive test and merely could be used after 1-2 weeks from the onset of the illness.
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Brief Report:
Analysis of the T354P mutation of the sodium/iodide cotransporter gene in children with congenital hypothyroidism due to dyshormonogenesis
Hajar Miranzadeh-Mahabadi, Modjtaba Emadi-Baygi, Parvaneh Nikpour, Neda Mostofizade, Silva Hovsepian, Mahin Hashemipour
Adv Biomed Res
2016, 5:73 (19 April 2016)
DOI
:10.4103/2277-9175.180642
PMID
:27169104
Background:
Congenital hypothyroidism (CH) due to the thyroid dyshormonogenesis is more prevalent in Iran in comparison to other countries. Sodium iodide symporter (
NIS
) is one of the plasma membrane glycoproteins that is located on the basolateral side of thyroid follicular cells and mediates active I
−
trapping into these cells. Playing a prominent role in thyroid hormone synthesis,
NIS
gene mutations can be a cause of permanent CH with the etiology of dyshormonogenesis. The aim of this study was to investigate the occurrence of T354P mutation of the
NIS
gene, in a group of children affected with permanent CH in Isfahan.
Materials and Methods:
Thirty-five patients with the etiology of dyshormonogenesis, and 35 healthy children, collected between 2002 and 2011 in Isfahan Endocrine and Metabolism Research Center, were examined for T354P mutation of the
NIS
gene by direct polymerase chain reaction-sequencing method.
Results:
No T354P mutation was detected in any of the studied children.
Conclusions:
More subjects with confirmed iodide transport defects should be screened for detecting the frequency of different reported
NIS
gene mutations in our population.
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Brief Report:
The correlation between anthropometric indices and hemodynamic changes after laryngoscopy and endotracheal intubation
Mohammadreza Safavi, Azim Honarmand, Elham Ghorbani Dasgerdi, Ghasem Mohammad Sharifi
Adv Biomed Res
2016, 5:45 (16 March 2016)
DOI
:10.4103/2277-9175.178805
PMID
:27110542
Background:
Cardiovascular hemodynamic changes after laryngoscopy and endotracheal intubations can cause serious complications. This study was carried out to evaluate the correlation between the anthropometric indices and hemodynamic changes after laryngoscopy and endotracheal intubation (EI).
Materials and Methods:
This descriptive–analytical pilot study was carried out in 2012, in the Kashani Hospital, Isfahan, Iran. After obtaining written informed consent from 130 patients who fulfilled the inclusion criteria, they were enrolled in the study. The recorded data included were, age, weight, height, neck circumference (NC), waist-to-hip ratio (W/H ratio) and body mass index (BMI). The heart rate (HR), systolic blood pressure (SAP), diastolic blood pressure (DBP), and mean arterial blood pressure (MAP) were recorded at baseline (before injection of the anesthetic drugs), just before laryngoscopy, and one, three, five, and ten minutes after EI.
Results:
The best cut-off points for BMI, NC, and W/H ratio, for prediction of significant cardiovascular changes after EI were, 26.56 kg/m
2
, 38 cm, and 0.82, respectively. There was a significant correlation between BMI and HR changes in the first and fifth minutes and also in MAP in the third and fifth minutes after EI (
P
< 0.05). Moreover, there was a significant correlation between NC and MAP in the fifth minute (
P
< 0.05). The W/H ratio was significantly related to the DBP in the tenth minute and MAP in the fifth and tenth minutes (
P
< 0.05).
Conclusions:
Based on the results of this study, among the anthropometric indices, the BMI, NC, and W/H ratio were significantly correlated with cardiovascular changes after laryngoscopy and tracheal intubation.
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Brief Report:
Phylogenetic analysis of otospiralin protein
Ibrahim Torktaz, Mohaddeseh Behjati, Amin Rostami
Adv Biomed Res
2016, 5:41 (16 March 2016)
DOI
:10.4103/2277-9175.178787
PMID
:27099854
Background:
Fibrocyte-specific protein, otospiralin, is a small protein, widely expressed in the central nervous system as neuronal cell bodies and glia. The increased expression of otospiralin in reactive astrocytes implicates its role in signaling pathways and reparative mechanisms subsequent to injury. Indeed, otospiralin is considered to be essential for the survival of fibrocytes of the mesenchymal nonsensory regions of the cochlea. It seems that other functions of this protein are not yet completely understood.
Materials and Methods:
Amino acid sequences of otospiralin from 12 vertebrates were derived from National Center for Biotechnology Information database. Phylogenetic analysis and phylogeny estimation were performed using MEGA 5.0.5 program, and neighbor-joining tree was constructed by this software.
Results:
In this computational study, the phylogenetic tree of otospiralin has been investigated. Therefore, dendrograms of otospiralin were depicted. Alignment performed in MUSCLE method by UPGMB algorithm. Also, entropy plot determined for a better illustration of amino acid variations in this protein.
Conclusion:
In the present study, we used otospiralin sequence of 12 different species and by constructing phylogenetic tree, we suggested out group for some related species.
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Brief Report:
Endothelial dysfunction in patients with subclinical hypothyroidism and the effects of treatment with levothyroxine
Nasmi Niknam, Noushin Khalili, Elham Khosravi, Mohsen Nourbakhsh
Adv Biomed Res
2016, 5:38 (16 March 2016)
DOI
:10.4103/2277-9175.178783
PMID
:27099851
Background:
Subclinical hypothyroidism (SHT) may increase the risk of cardiovascular disease. We compared endothelial function between SHT patients and euthyroid individuals, and evaluated the effects of levothyroxine therapy on endothelial function in the patients.
Materials and Methods:
In a quasi-experimental study, flow-mediated dilatation (FMD) and intima-media thickness (IMT) were assessed in SHT patients and healthy controls (
n
= 25 in each group). Patients then received levothyroxine (50 μg/day) for 2 months, and the FMD and IMT assessments were repeated.
Results:
Patients and controls were similar in IMT (0.56 ± 0.09 vs. 0.58 ± 0.08 mm,
P
= 0.481), but FMD was lower in patients than in controls (4.95 ± 2.02 vs. 6.50 ± 2.57%,
P
= 0.011). A significant increase was observed in FMD (4.11 ± 2.37%,
P
= 0.001), but not in IMT (−0.004 ± 0.020 mm,
P
= 0.327), after levothyroxine therapy among the patients.
Conclusions:
Patients with SHT have endothelial dysfunction which responds to levothyroxine therapy. Randomized placebo-controlled trials are needed to confirm these findings.
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Brief Report:
Effect of disease duration on personality type in multiple sclerosis patients and healthy individual
Sahar Vesal, Leila Dehghani, Masoud Etemadifar, Elahe Poorazizi, Sima Akhavan, Samaneh Mazrouei, Nasim Mehdizadeh, Zahra Saraf
Adv Biomed Res
2016, 5:35 (16 March 2016)
DOI
:10.4103/2277-9175.178807
PMID
:27099848
Background:
Multiple sclerosis may have profound emotional consequences. The relation between psychological and physical factors could lead patients toward unforeseen disease. This study focuses on multiple sclerosis (MS) disease duration on personality type A and B in relation to individuals' behaviors.
Materials and Methods:
This descriptive-analytical study was conducted in Isfahan Alzahra hospital in 2013. Three hundred MS patients and 100 healthy individuals were determined. The distributed questionnaires related to MS patients and considering the descriptive statistics such as demographic variables. Data were analyzed by SPSS software (version 18) based on Chi-square test and independent T-test.
Results:
Disease duration varied between 1 to 38 years: 30% (1-4 years), 38% (5-10 years), 20% (10-20 years), and 12% (more than 20 years). Significant relationship was observed between disease duration and tendency to type A (higher stress). This relation was positive and significant in Relapsing Remitting MS patients; but negative correlation was seen in Secondary Progressive MS patients. These patients tended to type B (lower stress) when disease duration increased.
Conclusions:
Individuals with disease duration of one year and less than one year tend to type A personality, while patients with increment of disease duration have tendency to type B.
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Brief Report:
Cytoprotective and antioxidant effects of human lactoferrin against H
2
O
2
-induced oxidative stress in human umbilical vein endothelial cells
Leila Safaeian, Shaghayegh Haghjoo Javanmard, Yaser Mollanoori, Nasim Dana
Adv Biomed Res
2015, 4:188 (31 August 2015)
DOI
:10.4103/2277-9175.164010
PMID
:26605227
Background:
Lactoferrin (LF) is an iron-binding glycoprotein with antioxidant, anti-inflammatory and nitric oxide-dependent vasodilatory properties. In the present study, we investigated the protective and antioxidant effects of LF on H
2
O
2
-induced oxidative stress in human umbilical vein endothelial cells (HUVECs).
Materials and Methods:
HUVECs were pretreated by (6.25–100 µg/ml) LF for 24 h and then exposed to 0.5 mM H
2
O
2
for 2 h. Cell viability was assessed by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The intra- and extra-cellular hydroperoxides concentration and ferric reducing antioxidant power (FRAP) were determined in pretreated cells.
Results:
Pretreatment of HUVECs with LF at the concentrations of 25–100 µg/ml significantly reduced the cytotoxicity of H
2
O
2
in a concentration-dependent manner using MTT assay. LF pretreatment at different concentration ranges also decreased the hydroperoxides level and augmented the FRA
P
value in both intra-and extra-cellular assay.
Conclusion:
These findings revealed antioxidant and cytoprotective effects of LF against H
2
O
2
-induced oxidative stress in HUVECs. With regard to the beneficial vascular activity of LF, further investigations are suggested for understanding its clinical value in human endothelial dysfunction and prevention and/or treatment of CVDs.
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Brief Report:
Risk factor assessment of stroke and its awareness among stroke survivors: A prospective study
K Shravani, Mihir Y Parmar, Ramyasri Macharla, Uday Venkat Mateti, Srinivas Martha
Adv Biomed Res
2015, 4:187 (31 August 2015)
DOI
:10.4103/2277-9175.164011
PMID
:26605226
Background:
Stroke is the second most common cause of death and major cause of disability worldwide. The objective of this study is to identify the major risk factors and assess the awareness among the stroke survivors.
Materials and Methods:
A prospective study was conducted at super specialty hospital, from December 2010 to July 2011. All the stroke patients of the age >25 years with either sex admitted in the hospital were included in the study. In order to assess the awareness among the stroke survivors, questionnaire established on the risk factors for stroke from the previously published studies.
Results:
A total of 100 patients with stroke or cerebrovascular accident were included in the study. Of 100 patients, 73% patients had ischemic stroke and 26% patients had hemorrhagic stroke. The mean age of the patients was 50 years and the incidence of stroke was predominant in males 73%, followed by females 27. It was observed that 70% of patients were hypertensives, 28% were diabetics, 27% were alcoholics, and 24% of patients had a habit of smoking, followed by others. The knowledge of the risk factors for stroke in stroke survivors was also very low, and the knowledge was varied among the subjects according to their level of educational status.
Conclusion:
This study reveals that hypertension is the most common risk factor for stroke followed by diabetes, smoking, and dyslipidemia. The awareness of risk factor among stroke survivors was poor.
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Brief Report:
Agreement between static magnetic resonance urography and diuretic renal scintigraphy in patients with ureteropelvic junction obstruction after pyeloplasty
Hamid Mazdak, Mehdi Karam, Fatemeh Ghassami, Alireza Malekpour
Adv Biomed Res
2015, 4:186 (31 August 2015)
DOI
:10.4103/2277-9175.164005
PMID
:26605225
Background:
Ureteropelvic junction obstruction (UPJO) is the most common cause of hydronephrosis within childhood that usually treat by surgery. According to anatomical variations in different individuals, scheduling similar procedures for all patients is not suitable, and thus the best decision for an appropriate surgical technique should be considered separately for each patient. Regardless of the type of applied technique, creating a funnel-shape UPJ with a suitable size is a successful treatment. In this context, the assessment of a successful surgical treatment in a short-term follow-up means repairing revealed anatomical defects. The present study aimed to compare the diagnostic value of static magnetic resonance urography (MRU) and diuretic-based renalscintigraphy (DRS) in patients with UPJO after pyeloplasty.
Materials and Methods:
A total of 30 consecutive patients with UPJO, who underwent unilateral pyeloplasty between 2012 and 2013 were assessed. All subjects underwent DRS and also MRU about 1-month after the former procedure.
Results:
The Kendall's tau correlation showed a very strong correlation between results of MRU and diuretic renal scintigraphy (
r
= 0.932,
P
< 0.001). This strong correlation was also shown by Somers'd test (
r
= 0.932,
P
< 0.001) similarly.
Conclusions:
Our study shows a strong agreement between DRS and MRU to assess UPJO. MRU static fluid has a high accuracy for assessment of renal system anatomy. Due to the lack of dangerous consequences of contrast materials, MRU can be the best option instead of DRS.
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Brief Report:
The relationship between quality of life and coping strategies in polycystic ovary syndrome patients
Leila Kolahi, Neda Asemi, Mohammadreza Mirzaei, Neda Adibi, Mina Beiraghdar, Asieh Maghami Mehr
Adv Biomed Res
2015, 4:168 (10 August 2015)
DOI
:10.4103/2277-9175.162545
PMID
:26436082
Background:
There are many factors that affect the quality of life, for example, stress and the coping strategies. Polycystic ovary syndrome is a common hormonal disorder leading to menstrual disorders, hirsutism, acne, obesity, infertility and abortion. In such cases, the patients suffer from a variety of stresses and face problems in their coping strategies with life's problems which can affect the quality of life and cause psychological distress and low the quality of life. The quality of life is a descriptive term which points to health and emotional, social and physical promotion of individuals as well as their ability to perform daily living tasks. The purpose of this study was to investigate the relationship between quality of life and coping strategies in patients with polycystic ovary syndrome.
Materials and Methods:
To perform this study, randomly 200 women who had inclusion criteria and were referred to Ali Shariati Hospital in Isfahan were selected and responded DLQI questionnaire and Carver coping strategies and form of demographic characteristics.
Results:
The mean score of quality of life in the patients was 4.14 ± 5.57. It was shown that acne has no effective role on quality of life and coping strategies in contrast in hirsute and non-hirsute patients; there was a significant difference in quality of life
P
value < 0.001). Also there is a significant relationship between the quality of life and coping strategies (problem solving, cognitive, emotional and social support) (
P
< 0.05) and quality of life has the highest correlation with emotional strategies (
r
= 0.46).
Conclusion:
According to results of this study, patients with PCOS are at risk pcychologicla disorders that may be led to decrease of quality of life. Thus this patients need to support by oppositions strategies. Also not only physical treatment but also psychological surveillance especially social support must be done for them.
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Brief Report:
Serum inflammatory markers in obese mice: Effect of ghrelin
Majid Khazaei, Zoya Tahergorabi
Adv Biomed Res
2015, 4:145 (27 July 2015)
DOI
:10.4103/2277-9175.161556
PMID
:26322293
Background:
Ghrelin is involved in modulation of food intake and energy homeostasis; however, it may play a role in cardiovascular system and atherosclerosis process. This study aimed to investigate the effect of ghrelin on serum inflammatory markers in control and obese mice.
Materials and Methods:
Ghrelin (100 mg/kg/day, twice daily) was administered interaperitoneally to control and diet-induced obese mice. After 10 days, blood samples were taken.
Results:
Results showed that administration of ghrelin did not change serum hsCRP level; however, it reduced serum IL-6 concentration in obese mice (
P
< 0.05).
Conclusion:
It seems that the exact role and mechanism of ghrelin in prevention or treatment of atherosclerosis needs more studies.
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Brief Report:
The effectiveness of lavender essence on strernotomy related pain intensity after coronary artery bypass grafting
Mohammad Ali Heidari Gorji, Om Golsum Ashrastaghi, Valiollah Habibi, Jamshid Yazdani, Mohammad Ebrahim Ebrahimzadeh, Mitra Ayasi
Adv Biomed Res
2015, 4:127 (4 June 2015)
DOI
:10.4103/2277-9175.158050
PMID
:26261829
Background:
Considering the side effects of pharmacological methods, there has been a suggestion to use nonpharmacological methods such Aromatherapy following coronary artery bypass grafting (CABG). This study aims to evaluate the effectiveness of lavender 2% aromatherapy on sternotomy pain intensity after coronary artery bypass graft surgery in patients who have undergone surgery.
Materials and Methods:
During this clinical trial, 50 patients who were candidates for CABG, were randomly divided into two equal groups, that is, the control group (
n
= 25) and the case group (
n
= 25). Following CABG, the case group received two drops of 2% lavender oil every 15 minutes with supplemental oxygen and the control group received only supplemental oxygen through a face mask. The data collection tools comprised of the demographic check list and visual analog scale (VAS) for evaluating the pain intensity. The pain intensity were assessed pre- and five, 30, and 60 minutes post aromatherapy. The final data were analyzed by the
t
-test and chi-squared test.
Results:
The findings showed that the pain perception intensity in the case group was lower than that in the control group at the 30- and 60-minute phases after intervention (
P
< 0.0001).
Conclusion:
The result indicated that aromatherapy can be used as a complementary method in postoperative pain reduction, as it reduced pain. The patients require two sedative drugs, and moreover, it avoids expenses of treatment.
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Brief Report:
Effects of aerobic exercise on plasma lipoproteins in overweight and obese women with polycystic ovary syndrome
Elaheh Abazar, Farzaneh Taghian, Farahnaz Mardanian, Dashti Forozandeh
Adv Biomed Res
2015, 4:68 (25 March 2015)
DOI
:10.4103/2277-9175.153892
PMID
:25878993
Background:
Polycystic ovary syndrome (PCOS) is one of the most frequent endocrine disorders. The PCOS manifest by hyperandrogenism, hypertension and cholesterol and lipoprotein improper profiles. Changing the life style, e.g. increasing physical activities is the first approach in controlling PCOS.
Materials and Methods:
Twenty-four women affected by polycystic ovary syndrome (PCOS) after medical screening were divided in to two groups: Experimental group (
n
= 12) and control group (
n
= 12), with the average age, weight, height, BMI and WHR of 26.87 ± 4.43 years, 75.71 ± 10.65 kg. 159.29 ± 6.44 cm, 29.86 ± 3.22 kg/m
2
and 91.75 ± 5.86 respectively. First the body composition such as BMI, WHR, percent body fat, weight and body fat mass were measured. In fasting blood samples the level of HDL, LDL, VLDL, triglyceride and cholesterol were measured. Then the experiment group underwent the effect of an aerobic exercise program. After 12 weeks, all the measured variables before intervention the test were re-measured. Correlated t-test was used for comparing the two groups before and after intervention the test and independent t-test was used for comparing the two groups (
P
< 0.05).
Results:
The results showed that after 12 weeks of exercise, BMI, WHR, fat rate, weight and fat mass and triglyceride had significant reduction and HDL had significant increase. But no significant changes happened in LDL, VLDL, and cholesterol levels.
Conclusion:
Reducing the weight by aerobic exercise in obese women and affected by PCOS can correct lipoprotein profile and improving health.
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Brief Report:
Detection of
Legionella
spp
.
by a nested-PCR assay in air samples of a wastewater treatment plant and downwind distances in Isfahan
Seyyed Abbas Mirzaee, Mahnaz Nikaeen, Yaghob Hajizadeh, BiBi Fatemeh Nabavi, Akbar Hassanzadeh
Adv Biomed Res
2015, 4:48 (17 February 2015)
DOI
:10.4103/2277-9175.151540
PMID
:25802817
Background:
Wastewater contains a variety of pathogens and bio -aerosols generated during the wastewater treatment process, which could be a potential health risk for exposed individuals. This study was carried out to detect
Legionella
spp. in the bio -aerosols generated from different processes of a wastewater treatment plant (WWTP) in Isfahan, Iran, and the downwind distances.
Materials and Methods:
A total of 54 air samples were collected and analyzed for the presence of
Legionella
spp. by a nested- polymerase chain reaction (PCR) assay. A liquid impingement biosampler was used to capture bio -aerosols. The weather conditions were also recorded.
Results:
Legionella
were detected in 6% of the samples, including air samples above the aeration tank (1/9), belt filter press (1/9), and 250 m downwind (1/9).
Conclusion:
The result of this study revealed the presence of
Legionella
spp. in air samples of a WWTP and downwind distance, which consequently represent a potential health risk to the exposed individuals.
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Brief Report:
The effect of estrogen on the expression of cartilage-specific genes in the chondrogenesis process of adipose-derived stem cells
Farzaneh Sadeghi, Ebrahim Esfandiari, Batool Hashemibeni, Fateme Atef, Hosein Salehi, Fateme Shabani
Adv Biomed Res
2015, 4:43 (11 February 2015)
DOI
:10.4103/2277-9175.151252
PMID
:25789269
Background:
During adolescence, sex hormones play an important role in regulating proliferation, differentiation, maturation, and the scheduled death of chondrocytes. Although some studies have reported the regulatory role of estrogen in the development and progression of cartilage, some of the mechanisms still remain unclear, including the role of estrogen in the expression of cartilage-specific genes in chondrogenesis process, which we cover in this study.
Materials and Methods:
In the present study, we used adipose-derived stem cells (ADSCs) to differentiate into cartilage. Differentiated cartilage cells were used in the control (without estrogen E2 in the culture medium) and experimental (with estrogen in the culture medium) groups to evaluate the expression of type II collagen and aggrecan as chondrogenic genes markers, with -real-time polymerase chain reaction technique.
Results:
Our results indicated that estrogen leads to inhibition of type II collagen gene expression and reduction of aggrecan gene expression.
Conclusion:
Therefore, estrogen probably has negative effects on chondrogenesis process of ADSCs.
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Brief Report:
Association of KCNJ11 (E23K) gene polymorphism with susceptibility to type 2 diabetes in Iranian patients
Ali Rastegari, Mohammad Rabbani, Hamid Mirmohammad Sadeghi, Elham Faghih Imani, Akbar Hasanzadeh, Fatemeh Moazen
Adv Biomed Res
2015, 4:1 (6 January 2015)
DOI
:10.4103/2277-9175.148256
PMID
:25625107
Background:
Type 2 diabetes (T2D) is a multifactorial disease with susceptibility of several genes that are related to T2D. Insulin secretion pathway starts with potassium channels in pancreatic beta cells. KCNJ11 gene encodes ATP-sensitive potassium channel subunits. Some studies suggested that KCNJ11 (E23K) mutation increases the risk of T2D. Therefore, present study was designed to investigate the association between E23K polymorphism of KCNJ11 gene and type 2 diabetes mellitus (T2DM) in the Iranian population.
Materials and Methods:
The type of study was case-control and 40 unrelated subjects, including 20 healthy controls and 20 diabetic patients were recruited (diagnosed based on American Diabetes Association criteria). Blood samples were used for isolation of genomic deoxyribonucleic acid (DNA). Having extracted the genomic DNA from human blood leukocytes by means of High Pure PCR Template Preparation Kit, PCR-restriction fragment length polymorphism method was used to detect KCNJ11 E23K gene polymorphism.
BanII
restriction enzyme was used for digestion. Data were analyzed using Chi-square or Fisher exact test or independent
t
-test, as appropriate.
P
< 0.05 was considered.
Results:
We found that the carrier homozygous for KK genotype are susceptible to T2D (0.049) and in patients the frequency of K allele was higher than control subjects (0.048).
Conclusion:
The present study suggests that KCNJ11 (E23K) gene polymorphism is associated with T2DM.
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Brief Report:
Knowledge, attitude, and performance of medical staff of teaching healthcare settings about hepatitis B and C in Isfahan, Iran
Behrooz Ataei, Mohsen Meidani, Maryam Khosravi, Farzin Khorvash, Mojtaba Akbari
Adv Biomed Res
2014, 3:267 (31 December 2014)
DOI
:10.4103/2277-9175.148249
PMID
:25625106
Background:
hospital personnel are at high risk of exposure, infection, and transmission of viral hepatitis. The present study aimed at investigating the knowledge, attitude, and performance of health service providers to provide them with the information required for their educational promotion on viral hepatitis.
Materials and Methods:
This cross-sectional study was conducted on 400 staff of the forenamed healthcare settings such as on nurses, midwives, licensed practical nurses, and lab officials in Isfahan, Iran, in 2012. A checklist including demographic data and questions associated with the knowledge (18 questions), attitude (4 questions), and performance (15 questions) on hepatitis B and C was completed by the participants.
Results:
A total of 388 participants completed the checklist. Participants' knowledge on the ways of transmission, prevalence, vaccination, and prevention methods was moderate (total score = 58.56 ± 10.1 percent) and the attitude was generally positive. Proper vaccination was carried out by 81.4% of the participants. Accidental injury by a needle was reported in 47.7% of the participants, but only 37.6% of them reported it to higher authorities and of them only 13.7% received appropriate treatment. Only 44.3% and 11.6% of participants reported always using gloves and masks, respectively, and 58.8% of the staff covered the needle cap before transferring it to the safety box.
Conclusions:
Due to excessive contact with patients, a paramedic-educated society is expected to have an optimal level of knowledge, attitude, and performance related to viral hepatitis. Our results from the checklists showed that medical personnel are not appropriately aware of viral hepatitis and their performance, too, is not satisfactory. Further continuous training is required and there needs to be more emphasis on actions regarding behaviors with high risk of infection transmission.
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Brief Report:
Resectability of the pancreatic adenocarcinoma: A study from Iran
Mahmud Baghbanian, Hasan Salmanroghani, Ali Baghbanian, Mohsen Bakhtpour, Bijan Shabazkhani
Adv Biomed Res
2014, 3:265 (31 December 2014)
DOI
:10.4103/2277-9175.148246
PMID
:25625104
Background:
Definite treatment of pancreatic adenocarcinoma is surgical resection. Absence of early symptoms in most patients leads to late diagnosis and treatment. This study aims to evaluate resectability of the pancreatic adenocarcinoma at the time of the diagnosis in Iran.
Materials and Methods:
The present study which is of a descriptive, prospective and case series nature, has been studying the resectability of the pancreatic adenocarcinoma by multi-detector computerized tomography, endoscopic ultrasonography, laparoscopy and/or laparotomy in 157 patients for the duration of 2 years since November 2009.
Results:
A total of 157 patients were enrolled in this study. Majority of them (68%) were male. The mean age was 67 years. Final diagnosis obtained 1-12 (2.7 ± 1.6) months after beginning of the symptoms. The lesion situated in the head of the pancreas in 127 cases (81%). Vascular invasion, lymphadenopathy, liver metastasis and peritoneal involvement were seen in 88%, 57%, 43% and 19% of the patients, respectively. According to imaging, tumor was resectable in 10 (6%) patients but laparoscopy and/or laparotomy revealed that only five cases (3%) were actually resectable. After 24 months, only 8 patients were alive; 5 cases of them had been treated by Whipple surgery and other 3 cases were under the chemotherapy. At 1 and 2 year survival rate of the patients was 11% and 5%, respectively.
Conclusion:
Majority (97%) of the pancreatic adenocarcinomas are unresectable at the time of diagnosis. Thus, meticulous preoperative assessment of patients is essential in patients to avoid major surgery in unresectable cases.
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Brief Report:
Comparison of Bristow procedure and Bankart arthroscopic method as the treatment of recurrent shoulder instability
Abolghasem Zarezade, Mohammad Dehghani, Ali Reza Rozati, Hossein Saeid Banadaki, Neda Shekarchizade
Adv Biomed Res
2014, 3:256 (12 December 2014)
DOI
:10.4103/2277-9175.146926
PMID
:25590034
Background:
Anterior shoulder dislocation is the most common major joint dislocation. In patients with recurrent shoulder dislocation, surgical intervention is necessary. In this study, two methods of treatment, Bankart arthroscopic method and open Bristow procedure, were compared.
Materials and Methods:
This clinical trial survey had been done in the orthopedic department of Alzahra and Kashani hospitals of Isfahan during 2008-2011. Patients with recurrent anterior shoulder dislocation who were candidates for surgical treatment were randomly divided into two groups, one treated by Bankart arthroscopic technique and the other treated by Bristow method. All the patients were assessed after the surgery using the criteria of ROWE, CONSTANT, UCLA, and ASES. Data were analyzed by SPSS software.
Results:
Six patients (16.22%) had inappropriate condition with ROWE score (score less than 75); of them, one had been treated with Bristow and five with Bankart (5.26 vs. 27.78). Nine patients (24.32%) had appropriate condition, which included six from Bristow group and three treated by Bankart technique (31.58 vs. 16.67). Finally, 22 patients (59.46%) showed great improvement with this score, which included 12 from Bristow and 10 from Bankart groups (63.16 vs. 55.56). According to Fisher's exact test, there were no significant differences between the two groups (
P
= 0.15).
Conclusion:
The two mentioned techniques did not differ significantly, although some parameters such as level of performance, pain intensity, use of analgesics, and range of internal rotation showed more improvement in Bristow procedure. Therefore, if there is no contraindication for Bristow procedure, it is preferred to use this method.
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Brief Report:
Molecular cloning of Reteplase and its expression in
E. coli
using tac promoter
Safieh Aghaabdollahian, Mohammad Rabbani, Kamran Ghaedi, Hamid Mir Mohammad Sadeghi
Adv Biomed Res
2014, 3:190 (12 September 2014)
DOI
:10.4103/2277-9175.140622
PMID
:25298959
Background and Aims:
This study aimed to clone and express the reteplase cDNA, a thrombolytic agent used for the treatment of acute myocardial infarction and stroke, in
E. coli
, utilizing tac promoter for its expression.
Materials and Methods:
Reteplase cDNA was amplified by polymerase chain reaction (PCR) with designed primers. The product was then cloned into pTZ57R plasmid
.
The cloned cDNA was digested out and ligated into pGEX-5x-1 expression vector. The presence of the insert was confirmed by restriction digestion. By using 0.2, 0.5 and 1 mM isopropyl beta-D thiogalactopyranoside (IPTG), expression of reteplase was induced in
E. coli TOP10
cells and analyzed by SDS-PAGE.
Results:
Electrophoresis of PCR product and also double digested recombinant pTZ57R plasmid, also, pGEX-5x-1 vector, showed a 1068bp band of reteplase. SDS-PAGE analysis showed a 60 KDa band of protein product induced with different concentrations of IPTG.
Conclusion:
In the present study, reteplase cDNA was successfully cloned and expressed using tac promoter. This vector will be used for the optimization of the expression of reteplase in
E. coli
.
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Brief Report:
Evaluation of effect of continuous positive airway pressure during cardiopulmonary bypass on cardiac de-airing after open heart surgery in randomized clinical trial
Mojtaba Mansour, Nasim Massodnia, Abolghasem Mirdehghan, Hamid Bigdelian, Gholamreza Massoumi, Zeinab Rafieipour Alavi
Adv Biomed Res
2014, 3:136 (28 May 2014)
DOI
:10.4103/2277-9175.133280
PMID
:24949307
Background:
Cardiac and pulmonary veins de-airing are of the most important steps during open heart surgery. This study evaluates the effect of continuous positive airway pressure (CPAP) on air trapping in pulmonary veins and on quality of de-airing procedure.
Materials and Methods:
This randomized prospective double blind clinical trial conducted on 40 patients. In the control group: During cardiopulmonary bypass (CPB), the ventilator was turned off and adjustable pressure limit (APL) valve was placed in SPONT position. In CPAP group: During CPB, after turning the ventilator off, the flow of oxygen flow was maintained at the rate of 0.5 L/min and the APL valve was placed in MAN position on 20 mbar. During cardiopulmonary bypass (CPB) weaning, the patients were observed for air bubbles in left atrium by using transesophageal echocardiography.
Results:
The mean de-airing time after the start of mechanical ventilation in CPAP group (
n
= 20) was significantly lower than the control group (
n
= 20) (
P
= 0.0001). The mean time of the left atrium air bubbles occupation as mild (
P
= 0.004), moderate (
P
= 0.0001) and severe (
P
= 0.015) grading was significantly lower in CPAP group.
Conclusions:
By CPAP at 20 mbar during CPB in open heart surgery, de-airing process can be down in better quality and in significantly shorter time.
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Brief Report:
New look at about nature, structure and function of Trietz ligament
Ghorbani Masoud, Sadeghi Hossain, Rashidi Bahman, Karimi Zeinab, Amouzegar Fatemeh
Adv Biomed Res
2014, 3:69 (27 January 2014)
DOI
:10.4103/2277-9175.125853
PMID
:24627877
Background:
Trietz ligament connects the duodeno-jejunal flexure to the right crus of the diaphragm. There are various opinions regarding the existence of the smooth muscle fibers in the ligament. We want to resolve this complexity with microscopic study of this part in cadavers.
Materials and Methods:
This study done on three cadavers in the medical faculty of Isfahan University of Medical Sciences. Three samples of histological specimens were collected from the upper, the central, and the lower parts of Trietz ligament and were stained by H and E staining and Mallory's trichrome stain. Three samples were collected from the regions of exact connection of the main mesentery to the body wall, the intestine, and the region between these two connected regions, and these specimens were stained.
Results:
In the microscopic survey, no collagen bundles were observed in the collected samples of the Trietz ligament after the dense muscular tissues. In the samples which were collected to work on collagen tissues stretching from the Trietz ligament to the main mesentery of intestine, no collagen bundles were observed.
Conclusion:
Trietz ligament is connected to the right crus of the diaphragm from the third and the fourth parts of the duodenum. Number of researchers state that there are smooth and striated muscular tissues and some others, with regard to observations of histological phases made from the samples of Trietz muscles, conclude that it can probably be noted that muscular bundles or the dense connective tissue bundles of collagen cannot be observed in the way we imagine.
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Extremely low-frequency electromagnetic field influences the survival and proliferation effect of human adipose derived stem cells
Shahnaz Razavi, Marzieh Salimi, Daryoush Shahbazi-Gahrouei, Saeed Karbasi, Saeed Kermani
Adv Biomed Res
2014, 3:25 (9 January 2014)
DOI
:10.4103/2277-9175.124668
PMID
:24592372
Background:
Extremely low-frequency electromagnetic fields (ELF-EMF) can effect on biological systems and alters some cell functions like proliferation rate. Therefore, we aimed to attempt the evaluation effect of ELF-EMF on the growth of human adipose derived stem cells (hADSCs).
Materials and Methods:
ELF-EMF was generated by a system including autotransformer, multi-meter, solenoid coils, teslameter and its probe. We assessed the effect of ELF-EMF with intensity of 0.5 and 1 mT and power line frequency 50 Hz on the survival of hADSCs for 20 and 40 min/day for 7 days by MTT assay. One-way analysis of variance was used to assessment the significant differences in groups.
Results:
ELF-EMF has maximum effect with intensity of 1 mT for 20 min/day on proliferation of hADSCs. The survival and proliferation effect (PE) in all exposure groups were significantly higher than that in sham groups (
P
< 0.05) except in group of 1 mT and 40 min/day.
Conclusion:
Our results show that between 0.5 m and 1 mT ELF-EMF could be enhances survival and PE of hADSCs conserving the duration of exposure.
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Investigating the relationship between intra-operative electrolyte abnormalities (sodium and potassium) with post-operative complications of coronary artery bypass surgery
Kaivan Bagheri, Mohammadreza Safavi, Azim Honarmand, Parviz Kashefi, Marziye Ghasemi, Leila Mohammadinia
Adv Biomed Res
2013, 2:82 (30 October 2013)
DOI
:10.4103/2277-9175.120871
Background:
Generally, the electrolyte abnormalities are seen in many hospitalized patients, and this problem increases in ones with heart diseases. The purpose of this study is determination of the prevalence of electrolyte abnormalities during the coronary artery bypass surgery (CABG) and detecting the relationship between these abnormalities with the complications after the surgeries.
Materials and Methods:
This is a cross-sectional study, which is done in Chamran hospital, the medical and educational center of Isfahan, Iran, in 2011. The target population included the patients who have undergone CABG in this hospital. In this study, 100 patients who had been candidates for CABG were selected, and we extracted their recorded intra-operative electrolyte information. The collected data was entered into the computer and analyzed by SPSS software. The Chi-square and t student tests were used for data analysis.
Results:
The mean ± SD of sodium during CABG was 137.95 ± 4.6 (range 127-152) mg\dl. Also, the mean ± SD of potassium was 4.65 ± 0.9 (range: 2.9-7.4). According to these results, 48 patients (48% of all) had electrolyte imbalance and 52 patients (52% of all) were normal. Sodium level in 71% of patients was normal, and in 29% of them was abnormal. Potassium level in 73% of individuals was normal, and in 27% of them was abnormal.
Conclusion:
Giving an attention to electrolyte abnormalities in patients who have undergone CABG surgery is a considerable necessity for them, and sufficient arrangements are needed to prevent such abnormalities.
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Bladder mucosal autograft: An effective method for repair of vesicovaginal fistula
Mohammad G Mohseni, Seyed R Hosseini, Farshid Alizadeh, Shahram Gooran, Nadia Valikhani
Adv Biomed Res
2012, 1:77 (31 October 2012)
DOI
:10.4103/2277-9175.102984
PMID
:23326807
Background:
to investigate the effectiveness of bladder mucosal autograft for the treatment of vesicovaginal fistulae.
Materials and Methods:
Between March 2005 and June 2011, 21 patients with a single vesicovaginal fistula above the trigone, not involving the ureters, underwent surgery. Bladder was approached extraperitoneally and opened in the midline. The mucosa around the fistula was incised and inverting sutures were placed over the fistula opening. The mucosal defect was covered by a free mucosal graft from the edge of cystotomy incision.
Results:
After catheter removal at 2 weeks, 18 patients (85 %) remained dry while one patient experienced urge incontinence, which resolved in a few days and another one still had urine leakage (although less than before the operation) that improved after another 3 weeks of bladder drainage. Only in one patient, the operation failed.
Conclusion
: Short duration of hospitalization, simplicity of the procedure, avoidance of extensive bladder dissection, and extraperitoneal nature of the operation, along with a high success rate are the advantages of this procedure. This technique could be recommended for single fistulae not involving the ureters and not secondary to malignancies.
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