ORIGINAL ARTICLE
Year : 2023 | Volume
: 12 | Issue : 1 | Page : 54-
Toll-like receptor 7 a novel non-invasive inflammatory genetic sensor for ulcerative colitis remission monitoring
Hamid Asadzadeh-Aghdaei1, Leili Rejali1, Mahyar Nourian2, Vahid Chaleshi1, Naghmeh Zamani1, Shaghayegh Baradaran-Ghavami1, Mohsen Nemati1, Shabnam Shahrokh1, Mohsen Norouzinia3, Massoud Vosough4, Ehsan Nazemalhosseini-Mojarad3, Mohammadreza Zali3
1 Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2 Mahak Hematology Oncology Research Center (Mahak-HORC), Mahak Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3 Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4 Department of Regenerative Medicine, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
Correspondence Address:
Dr. Ehsan Nazemalhosseini-Mojarad
Gastroenterology, and Liver Disease Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Yeman St, Chamran Expressway, Thran
Iran
Background: Ulcerative colitis (UC) and Crohn's disease (CD) are two major types of inflammatory bowel diseases (IBDs). Toll-like receptors (TLRs) are expressed in the innate immune system compartments, in charge of identifying a wide range of microorganisms. The aim of the present study was to evaluate the expression of TLR-2, -7, and -8 in peripheral blood mononuclear cells (PBMC) of UC patients as a novel non-invasive primary inflammation sensor for monitoring the clinical course of UC candidates.
Materials and Methods: In this cross-sectional study, total RNA was extracted from the PBMC of 42 UC patients along with 20 healthy donors. The mRNA levels of TLR-2, -7, and -8 were assessed using the quantitative real-time polymerase chain (qRT-PCR) reaction.
Results: The present research study demonstrated no significant changes in TLR-2 mRNA expression in UC patients in comparison with the control group (P = 0.1264), whereas significant elevation (P = 0.0008) was distinguished in the TLR-7 expression of UC participants specifically during the remission course compared with healthy donors and flareup patients (P = 0.0004 and P = 0.0063, respectively). The last selected TLR, TLR-8 was not shown remarkable changes either between UC patients and the control group or between clinical courses of the disease.
Conclusion: Here, among three nominated TLRs for predicting UC patients, TLR-7 was potentially selected according to the significant difference in mRNA expression in flareup UC patients and control donors. TLR-7 could be used as a novel non-invasive biomarker for monitoring UC patients in the active course of the disease.
How to cite this article:
Asadzadeh-Aghdaei H, Rejali L, Nourian M, Chaleshi V, Zamani N, Baradaran-Ghavami S, Nemati M, Shahrokh S, Norouzinia M, Vosough M, Nazemalhosseini-Mojarad E, Zali M. Toll-like receptor 7 a novel non-invasive inflammatory genetic sensor for ulcerative colitis remission monitoring.Adv Biomed Res 2023;12:54-54
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How to cite this URL:
Asadzadeh-Aghdaei H, Rejali L, Nourian M, Chaleshi V, Zamani N, Baradaran-Ghavami S, Nemati M, Shahrokh S, Norouzinia M, Vosough M, Nazemalhosseini-Mojarad E, Zali M. Toll-like receptor 7 a novel non-invasive inflammatory genetic sensor for ulcerative colitis remission monitoring. Adv Biomed Res [serial online] 2023 [cited 2023 Mar 27 ];12:54-54
Available from: https://www.advbiores.net/article.asp?issn=2277-9175;year=2023;volume=12;issue=1;spage=54;epage=54;aulast=Asadzadeh-Aghdaei;type=0 |
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